SULFUR OXIDATION IN C ELEGANS BY OXIDATIVE STRESS AS COMPARED TO THE AGING PROC

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 秀丽隐杆线虫作为模式生物，由于其寿命短且易于控制交配，非常适合衰老研究。雅各布斯实验室在秀丽隐杆线虫和衰老方面进行了广泛的研究，最近致力于研究氧化应激的作用。他们与其他人一起开发了 OxICAT 系统，用于绘制氧化还原活性硫醇蛋白质组图谱。他们的结论表明，虽然氧化应激和衰老都会导致可逆氧化半胱氨酸残基水平相当，但目标蛋白却不同。然而，他们目前的方法只能绘制氧化为二硫化物的半胱氨酸，而不能绘制氧化为磺酸或磺酸的半胱氨酸。与 Jakobs 实验室合作，我们建议使用硫 XANES 来绘制总硫氧化态以及与氧化应激相比，由于老化过程而发生的不可逆硫氧化的量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. C. elegans as a model organism is well suited for studies in aging due to its short life span and easily controlled mating. The Jakobs lab has worked extensively on C. elegans and aging, most recently working on the role of oxidative stress. They, with other have developed the OxICAT system for mapping the redox active thiol proteome. Their conclusions show that while oxidative stress and aging both lead to comparable levels of reversibly oxidized cysteine residues, target proteins are different. However, their current methods can only map cysteines oxidized to disulphides and not cysteines oxidized to sulfunic or sulfonic acids. In conjunction with the Jakobs lab, we propose to use Sulfur XANES to map the total sulfur oxidation state and the amount of irreversible sulfur oxidation that happens as a result of the aging process compared to that of oxidative stress.