SULFUR OXIDATION IN C ELEGANS BY OXIDATIVE STRESS AS COMPARED TO THE AGING PROC

与老化过程相比，线虫中氧化应激引起的硫氧化

• 批准号: 8362222
• 负责人: JAY P STASSER
• 金额: $ 0.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362222
• 关键词: Aging; Aging-Related Process; Animal Model; Caenorhabditis elegans; Cysteine; Disulfides; Funding; Grant; Lead; Longevity; Maps; Methods; National Center for Research Resources; Oxidation-Reduction; Oxidative Stress; Partner in relationship; Principal Investigator; Proteins; Proteome; Radiation; Research; Research Infrastructure; Resources; Role; Source; Sulfhydryl Compounds; Sulfonic Acids; Sulfur; System; United States National Institutes of Health; Work; cost; oxidation; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. C. elegans as a model organism is well suited for studies in aging due to its short life span and easily controlled mating. The Jakobs lab has worked extensively on C. elegans and aging, most recently working on the role of oxidative stress. They, with other have developed the OxICAT system for mapping the redox active thiol proteome. Their conclusions show that while oxidative stress and aging both lead to comparable levels of reversibly oxidized cysteine residues, target proteins are different. However, their current methods can only map cysteines oxidized to disulphides and not cysteines oxidized to sulfunic or sulfonic acids. In conjunction with the Jakobs lab, we propose to use Sulfur XANES to map the total sulfur oxidation state and the amount of irreversible sulfur oxidation that happens as a result of the aging process compared to that of oxidative stress.

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