AZITHROMYCIN ATTENUATION OF INFLAMMATION IN A NON-INFECTIOUS ASTHMA

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 关于大环内酯类抗生素治疗哮喘的抗炎作用的结论很难确定，因为它们的有益作用可能与它们的抗菌作用有关。 我们假设阿奇霉素具有独特的抗炎特性，并在非感染性过敏性哮喘小鼠模型中验证了这一假设。 为了诱导过敏性气道炎症，7周龄BALB/cJ小鼠在第0天和第7天进行腹腔内卵清蛋白致敏，然后在第14天进行鼻内激发。在第13至16天用PBS或阿奇霉素处理小鼠，并在第17天通过定量气道中的白细胞、支气管肺泡灌洗液（BAL）中多种炎性介质的表达和粘液细胞化生来评估气道炎症。在一组单独的实验中，在卵清蛋白攻击后开始阿奇霉素或PBS处理。每个实验重复3次（每组总共9-11只小鼠）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Definitive conclusions regarding the anti-inflammatory effects of macrolide antibiotics for treatment of asthma are difficult to formulate since their beneficial effects may be related to their antimicrobial action. We hypothesized that azithromycin possesses distinct anti-inflammatory properties and tested this assumption in a non-infectious mouse model of allergic asthma. To induce allergic airway inflammation seven-week-old BALB/cJ mice underwent intraperitoneal ovalbumin sensitization on days 0 and 7 followed by intranasal challenge on day 14. Mice were treated with PBS or azithromycin on days 13 through 16 and on day 17 airway inflammation was assessed by quantifying leukocytes in the airway, expression of multiple inflammatory mediators in the bronchoalveolar lavage (BAL), and mucous cell metaplasia. In a separate set of experiments, azithromycin or PBS treatment were initiated after the ovalbumin challenge. Each experiment was repeated 3 times (a total of 9-11 mice in each group).