EXAFS COMPARISON OF P450-PEROXYNITRITE INTERMEDIATES AND P450-NO COMPLEXES

P450-过氧亚硝酸盐中间体和 P450-NO 络合物的 EXAFS 比较

• 批准号: 8362407
• 负责人: MICHAEL T. GREEN
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362407
• 关键词: Advocate; Complex; Cytochrome P450; Enzymes; Family; Funding; Grant; Lead; National Center for Research Resources; Peroxonitrite; Principal Investigator; Radiation; Raman Spectrum Analysis; Reporting; Research; Research Infrastructure; Resources; Source; Techniques; United States National Institutes of Health; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recently, the use of peroxynitrite (PN) has been advocated as a means of generating high-valent P450 intermediates. This technique has lead to a number of reports that question our understanding of this large family of enzymes. We wish to use EXAFS, paired with M¿ssbuaer and Raman spectroscopies, to provide a definitive characterization of the P450-PN intermediate.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 最近，过氧亚硝酸根(PN)的使用被认为是产生高价P450中间体的一种手段。这项技术已经导致了一些报告，质疑我们对这一大家族酶的理解。我们希望使用EXAFS结合M？ssbuaer和拉曼光谱来提供对P450-PN中间体的明确表征。