SMALL ANGLE SOLUTION X-RAY SCATTERING STUDIES ON METALLOPROTEINS

金属蛋白质的小角解X射线散射研究

• 批准号: 8362331
• 负责人: JOERG GUENTER GROSSMAN
• 金额: $ 0.14万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362331
• 关键词: Complex; Cuprozinc Superoxide Dismutase; Data; Enzymes; Funding; Genome; Grant; Imagery; Individual; Metalloproteins; Metals; Molecular Conformation; National Center for Research Resources; Neurodegenerative Disorders; Pathway interactions; Physiological; Play; Predisposition; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resolution; Resources; Roentgen Rays; Role; Shapes; Solutions; Source; Structure; United States National Institutes of Health; Work; cost; denitrification; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. More than half of the proteins in a genome contain metals, which have catalytic and/or structural roles. They therefore play a significant part in a considerable number of physiological functions. This proposal primarily focuses on the visualization of conformations and structural changes of (i) Cu-enzymes and their complexes of the denitrification pathway and (ii) proteins involved in neurodegenerative diseases due to their susceptibility to aggregate, here with a particular emphasis on Cu, Zn superoxide dismutase. In the absence of any structural information SAXS can provide valuable ab initio 3D shapes at low to medium resolution. Yet our complementary crystallographic work will allow us to exploit the scattering data meaningfully in particular in view of conformational changes or associations of molecules whose individual structures are known.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 基因组中超过一半的蛋白质含有金属，这些金属具有催化和/或结构作用。因此，它们在相当多的生理功能中发挥重要作用。该建议主要集中在可视化的构象和结构变化的（i）铜酶及其复合物的反硝化途径和（ii）蛋白质参与神经退行性疾病，由于其易感性聚集，在这里特别强调铜，锌超氧化物歧化酶。在没有任何结构信息的情况下，SAXS可以在低到中等分辨率下提供有价值的从头算3D形状。然而，我们的互补晶体学工作将使我们能够利用散射数据有意义的，特别是鉴于构象变化或协会的分子，其个别结构是已知的。