CARDIAC PHENOTYPING OF ETS-1 KNOCKOUT MICE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 对ETS-1 KO小鼠进行全面的心脏表型分析，并将表型与在人类染色体疾病Jacobsen综合征（11 q末端缺失障碍）中观察到的表型进行比较。 具体来说，我非常有兴趣与您合作，让您对我的基因工程ETS-1敲除小鼠进行心脏MRI分析。我们已经证明他们有很大的室间隔缺损，我很有兴趣通过三维核磁共振分析来确定他们是否有其他的心脏缺损。 我希望分析能够包括冠状动脉和肺静脉的检查。 大多数KO小鼠具有较大的室间隔缺损（通过组织病理学分析）。 我们希望使用MRI更全面地定义这些胚胎的三维解剖结构，包括理想的冠状动脉和肺静脉解剖结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To perform comprehensive cardiac phenotyping of ETS-1 KO mice, and compare the phenotypes to those observed in the human chromosomal disorder Jacobsen syndrome (11q Terminal Deletion Disorder) Specifically, I am very interested in collaborating with you to have you perform cardiac MRI analysis of my genetically engineered ETS-1 knockout mice. We have already shown that they have large ventricular septal defects, and I am very interested in determining, through 3-D MRI analysis, whether they might have any additional heart defects. I hope the analysis will be able to include an examination of the coronary arteries and pulmonary veins. Most of the KO mice have large ventricular septal defects (by histopathologic analysis). We want to use MRI to more comprehensively define the 3-D anatomy of these embryos, including ideally coronary artery and pulmonary vein anatomy.