STRUCTURAL STUDIES OF NUCLEIC ACID PROCESSING ENZYMES

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们目前正在研究许多蛋白质结构域参与DNA和RNA连接的性质。 这些结构域包括细菌和古细菌LigD蛋白的磷酸酯酶结构域、细菌和古细菌RNA修复系统的连接酶组分PnkP/Hen 1以及细菌3 '-PO 4连接途径（以RtcB酶为例）。 此外，我们还得到了E.大肠杆菌PrrC tRNA核酸酶，一种新的核糖毒素（详情如下）。 有关代表性工作，请参阅以下出版物（所有工作均在NSLS进行）：PMID：21208981 PMID：20616014 PMID：20399182

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are currently investigating properties of numerous protein domains involved in DNA and RNA ligation. Among these domains are the phosphoesterase domains of bacterial and archaeal LigD proteins, the ligase components of bacterial and archaeal RNA repair systems PnkP/Hen1, and the bacterial 3'-PO4 ligation pathway exemplified by the RtcB enzyme. In addition, we have diffracting crystals of E. coli PrrC tRNA nuclease, a novel ribotoxin (details below). Consult the following publications for representative work (all of which was carried out at the NSLS):PMID: 21208981PMID: 20616014PMID: 20399182