MASS SPECTROMETRY OF SIGNALLING LIPIDS

信号脂质的质谱分析

• 批准号: 8365572
• 负责人: Barbara A Baird
• 金额: $ 0.46万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365572
• 关键词: Biochemical; Biology; Cell membrane; Data; Funding; Grant; IgE Receptors; Lipids; Mass Spectrum Analysis; Medicine; National Center for Research Resources; Paper; Phosphatidylinositol 4,5-Diphosphate; Phosphatidylinositols; Principal Investigator; Publishing; Research; Research Infrastructure; Research Personnel; Resources; Signal Transduction; Source; Students; United States National Institutes of Health; Visit; Work; cost; inorganic phosphate; interest; receptor-mediated signaling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The collaborators would like to carry out mass spectrometric of cell signaling lipids. They are evaluating phosphatidyl inositol bis phosphate (PIP2) distributions in plasma membranes related to IgE receptor mediated signaling. They have already obtained some preliminary data and have published a paper with Prof. McLafferty, but this are of research is outside of his usual research, so he has referred the investigators to the BUSM Resource. The approach theywould like to use parallels that of some published work, but the difficulty of the work proposed here is greater. The primary result they seek is correlating their present biochemical data to certain peaks observed in the mass spectra. It also should be very interesting to quantify different PIP2 species. Some initial data has been obtained at BUSM and the Cornell student has visited to participate in further analyses.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 合作者希望对细胞信号脂质进行质谱分析。他们正在评估与 IgE 受体介导的信号传导相关的质膜中磷脂酰肌醇二磷酸 (PIP2) 的分布。他们已经获得了一些初步数据，并与 McLafferty 教授一起发表了一篇论文，但这项研究超出了他的日常研究范围，因此他将研究人员推荐给了 BUSM Resource。他们希望使用的方法与一些已发表的工作类似，但这里提出的工作难度更大。他们寻求的主要结果是将他们当前的生化数据与质谱中观察到的某些峰相关联。量化不同的 PIP2 物种也应该非常有趣。 BUSM 已获得一些初步数据，康奈尔大学的学生已前往参与进一步分析。