RNA STRUCTURE AND FUNCTION

RNA结构和功能

• 批准号: 8361700
• 负责人: Navtej Singh Toor
• 金额: $ 0.55万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361700
• 关键词: Catalytic Domain; Catalytic RNA; Complex; Crystallography; Eukaryota; Excision; Exons; Funding; Grant; Human Genome; Introns; Ligation; National Center for Research Resources; Nuclear; Principal Investigator; Process; RNA; RNA Splicing; Research; Research Infrastructure; Resources; Retroelements; Source; Spliceosomes; Structure; United States National Institutes of Health; cost; genetic element; mRNA Precursor; protein complex; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our research involves studying the structure and function of large catalytic RNAs and RNA-protein complexes using x-ray crystallography. Specifically, we are studying group II introns, which are a class of self-splicing ribozymes that can also function as mobile retroelements. Group II introns undergo a two-step splicing process that leads to their excision from pre-mRNAs and results in the ligation of the adjacent exons. They are also thought to be the ancestors of the spliceosomal introns and non-LTR retroelements, which together comprise ~50% of the human genome. The group II intron and the spliceosome also share a conserved, catalytic core. The spliceosome is a multi-megadalton complex which is responsible for excising nuclear introns in eukaryotes. Through studying group II introns, we can also gain an understanding of these more evolutionarily advanced genetic elements.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们的研究包括利用X射线结晶学研究大的催化RNA和RNA-蛋白质复合体的结构和功能。具体地说，我们正在研究第二组内含子，这是一类自我剪接的核酶，也可以作为可移动的逆转录元件。第二组内含子经历两步剪接过程，导致它们从前mRNAs中被切除，并导致相邻外显子的连接。它们也被认为是剪接体内含子和非LTR逆转录元件的祖先，这两种元件加起来占人类基因组的50%左右。 第二类内含子和剪接体也共享一个保守的催化核心。剪接体是一个多兆吨的复合体，负责切除真核生物中的核内含子。通过研究第二组内含子，我们还可以了解这些进化更先进的遗传元件。