REPLICATIVE AGING IN YEAST USING AN ENGINEERED DAUGHTER ARRESTER STRAIN

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 在这里，我们建议使用NCXT光束线研究工程菌株的形态变化，以丰富在旧的酿酒酵母细胞。这项研究将提供酵母老化的形态表型的第一个全面的看法。 我们建议在存在和不存在抑制性化合物的情况下，检查父母和女儿避雷器菌株的形态。虽然引入子arrester基因或药物的存在相对于不存在药物的亲本菌株不会显著影响细胞周期长度，但任何一种变化都可能导致细微的形态学改变。我们建议使用软X射线断层扫描定量比较酵母细胞形态的这些菌株在这些不同的条件下，以检测这种变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Here we propose to use the NCXT beamline to study morphological changes of a strain engineered to be enriched in old Saccharomyces cerevisiae cells. This study will provide a first comprehensive view of the morphological phenotypes of aging in yeast. We propose to examine the morphology of the parent and daughter arrester strains in both in the presence and absence of an inhibitory compound. While the introduction of the daughter arrester gene or presence of drug does not significantly affect the cell cycle length relative to the parent strain in the absence of drug, it is possible that either change could cause subtle morphology alterations. We propose to use soft X-ray tomography to quantitatively compare the yeast cell morphologies of these strains under these different conditions to detect such changes.