MULTIFREQUENCY ESR STUDIES OF TAR DNA DYNAMICS

TAR DNA 动力学的多频 ESR 研究

• 批准号: 8364089
• 负责人: Charles P. Scholes
• 金额: $ 0.28万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364089
• 关键词: DNA; Funding; Future; Grant; HIV-1; National Center for Research Resources; Nucleocapsid Proteins; Play; Principal Investigator; Protein Binding; RNA; RNA Probes; Research; Research Infrastructure; Resources; Response Elements; Role; Source; Spin Labels; Structure; Technology; Transactivation; United States National Institutes of Health; Virus Replication; cost; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The HIV-1 Nucleocapsid protein 7 (NCp7) is a small protein that binds to the stem-loop structures of both DNA and RNA. The recognition and folding of NCp7 and DNA/RNA stem loops play an important role in virus replication.The high field ESR was applied in addition to the X-band ESR to investigate the dynamics of Transactivation Response Element (TAR) DNA stem loop and its interaction with NCp7. The nixroxide spin labels were attached to the stem, loop, and bulge regions of TAR DNA, as well as to the 5' and 3' terminals of the DNA. Future studies to compare the multifrequency spectra of complementary TAR DNA and TAR RNA probed by spin labels in the stem, loop, and bulge regions were planned to tell the difference between the dynamics of complementary TAR DNA and TAR RNA.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 HIV-1核衣壳蛋白7(NCp7)是一种与DNA和RNA的茎环结构结合的小蛋白。NCp7和DNA/RNA茎环的识别和折叠在病毒复制过程中起着重要作用，除X波段ESR外，还应用高场ESR研究了反式激活反应元件(TAR)DNA茎环的动力学及其与NCp7的相互作用。氮氧化物自旋标记贴在TAR DNA的茎、环和凸起区域，以及DNA的5‘和3’末端。未来计划进行比较互补TAR DNA和TAR RNA的多频谱研究，以区分互补TAR DNA和TAR RNA的动力学差异。