Surface Proteins in the Differentiation of Stem Cells to Cardiomyocytes
干细胞向心肌细胞分化中的表面蛋白
基本信息
- 批准号:8249072
- 负责人:
- 金额:$ 24.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-04-01 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAmericanAnalytical BiochemistryAnimalsAttentionCardiacCardiac MyocytesCardiovascular systemCell LineCell SeparationCell Surface ProteinsCell surfaceCellsClinicDevelopmentEmbryonic DevelopmentEnvironmentEventGoalsHeartHeart DiseasesHeart failureIn VitroInstitutionInstructionKnowledgeLifeMass Spectrum AnalysisMembrane ProteinsMentorsMethodsModelingMolecularMuscle CellsMyocardial InfarctionPopulationProliferatingProteinsQuality of lifeRegenerative MedicineResearchResearch PersonnelSpecificityStagingStem cellsStrokeTimeVentricularWorkbasecell typecellular developmentclinically relevantdesignhigh riskimprovedin vivoinstrumentationnovelprogenitorrepairedstem cell differentiationstem cell populationsuccess
项目摘要
Heart disease is the single largest killer of Americans. Those that survive a heart attack have a higher risk of
another attack, heart failure, and stroke. Consequently, there is a need to develop approaches for repairing
the heart and improving the quality of life after a heart attack. The concept of regenerative medicine through
the use of stem cells is gaining attention as a potential therapy for restoring cardiac function. Hurdles
currently faced in the development of cellular therapies for the clinic are (1) the limited availability of cell
surface markers for selecting pure cell populations and for tracking differentiation and (2) the lack of
knowledge of how in vitro differentiation and in vivo development of cardiomyocytes correlate. The long term
goal of this project is to characterize and understand stem cell differentiation. We aim to achieve this by
identifying and characterizing specific cell surface protein 'barcodes' useful for marking specific cell types.
The major objectives are to first address the need for more cell surface markers that are useful for
characterizing specific cell types, stages, and lineages, and specifically, we will focus on pluripotent cells,
cardiac progenitors, and ventricular cardiomyocytes. We will use state-of-the-art analytical biochemistry and
mass spectrometry approaches to identify and quantify novel surface proteins in a model cell line and these
proteins will then be evaluated for their utility in live cell sorting to obtain pure, defined populations.
Subsequently, we will apply the knowledge gained from the in vitro work to understanding cardiac cell
development in the animal. Specifically, we will determine whether the in vitro markers are also present at
the appropriate time and space during embryonic development. The specific aims are designed to develop
an understanding of the function of the cell surface markers and whether the molecular events during in vitro
differentiation mimic those during in vivo development of cardiomyocytes. The supportive environment at my
new institution will facilitate the continued success of this proposal by providing access to state-of-the art
instrumentation, mentors with specialized expertise, and the forum to support discussion of clinically
relevant research.
心脏病是美国人最大的单一杀手。那些在心脏病发作中幸存下来的人有更高的风险
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Rebekah L. Gundry其他文献
SurfaceGenie: A web-based application for prioritizing cell-type specific marker candidates
SurfaceGenie:基于网络的应用程序,用于优先考虑细胞类型特定标记候选物
- DOI:
10.1101/575969 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
M. Waas;S. Snarrenberg;J. Littrell;Rachel A. Jones Lipinski;P. Hansen;J. Corbett;Rebekah L. Gundry - 通讯作者:
Rebekah L. Gundry
Correction to: Importance of evaluating protein glycosylation in pluripotent stem cell‑derived cardiomyocytes for research and clinical applications
- DOI:
10.1007/s00424-021-02566-7 - 发表时间:
2021-06-01 - 期刊:
- 影响因子:2.900
- 作者:
Maia I. Kelly;Mustafa Albahrani;Chase Castro;Ellen Poon;Bin Yan;Jack Littrell;Matthew Waas;Kenneth R. Boheler;Rebekah L. Gundry - 通讯作者:
Rebekah L. Gundry
Cardiomyocyte Differentiation Promotes Cell Survival During Nicotinamide Phosphoribosyltransferase Inhibition Through Increased Maintenance of Cellular Energy Stores
烟酰胺磷酸核糖转移酶抑制期间心肌细胞分化通过增加细胞能量储存的维持来促进细胞存活
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:6
- 作者:
E. Kropp;Katarzyna A. Broniowska;M. Waas;Alyssa Nycz;J. Corbett;Rebekah L. Gundry - 通讯作者:
Rebekah L. Gundry
The Albuminome as a Tool for Biomarker Discovery
蛋白组作为生物标志物发现的工具
- DOI:
- 发表时间:
2008 - 期刊:
- 影响因子:0
- 作者:
Rebekah L. Gundry;R. Cotter - 通讯作者:
R. Cotter
INACTIVATION OF MANGANESE SUPEROXIDE DISMUTASE BY IRREVERSIBLE COVALENT OXIDATIVE MODIFICATION IN CARDIOVASCULAR DISEASE
- DOI:
10.1016/s0735-1097(20)31665-x - 发表时间:
2020-03-24 - 期刊:
- 影响因子:
- 作者:
Daniel R. Anderson;Michael Duryee;Jake Walker;Johnathan H. Hall;Geoffrey M. Thiele;Lynell Klassen;Matt Zimmerman;Rebekah L. Gundry;Dahn L. Clemens - 通讯作者:
Dahn L. Clemens
Rebekah L. Gundry的其他文献
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{{ truncateString('Rebekah L. Gundry', 18)}}的其他基金
Harnessing Glycoproteomics and Glycomics to Understand Cardiac Biology and Disease
利用糖蛋白组学和糖组学来了解心脏生物学和疾病
- 批准号:
10337288 - 财政年份:2021
- 资助金额:
$ 24.27万 - 项目类别:
Harnessing Glycoproteomics and Glycomics to Understand Cardiac Biology and Disease
利用糖蛋白组学和糖组学来了解心脏生物学和疾病
- 批准号:
10555323 - 财政年份:2021
- 资助金额:
$ 24.27万 - 项目类别:
Development of a next-generation glycomics platform to enable glycan structure analyses for precision medicine
开发下一代糖组学平台,以实现精准医学的聚糖结构分析
- 批准号:
10054508 - 财政年份:2020
- 资助金额:
$ 24.27万 - 项目类别:
Development of a next-generation glycomics platform to enable glycan structure analyses for precision medicine
开发下一代糖组学平台,以实现精准医学的聚糖结构分析
- 批准号:
10239250 - 财政年份:2020
- 资助金额:
$ 24.27万 - 项目类别:
Cell Surface Phenotyping Human Primary Cells
人类原代细胞的细胞表面表型分析
- 批准号:
10034909 - 财政年份:2019
- 资助金额:
$ 24.27万 - 项目类别:
Surface Proteins in the Differentiation of Stem Cells to Cardiomyocytes
干细胞向心肌细胞分化中的表面蛋白
- 批准号:
8449291 - 财政年份:2011
- 资助金额:
$ 24.27万 - 项目类别:
Surface Proteins in the Differentiation of Stem Cells to Cardiomyocytes
干细胞向心肌细胞分化中的表面蛋白
- 批准号:
8166085 - 财政年份:2011
- 资助金额:
$ 24.27万 - 项目类别:
Surface Proteins in the Differentiation of Stem Cells to Cardiomyocytes
干细胞向心肌细胞分化中的表面蛋白
- 批准号:
7572060 - 财政年份:2009
- 资助金额:
$ 24.27万 - 项目类别:
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