The Hsp90 molecular chaperone system

Hsp90分子伴侣系统

基本信息

项目摘要

DESCRIPTION (provided by applicant): Our long-term objective is to understand the cellular breadth and impact of the Hsp90 molecular chaperone system. To dissect this large and diverse chaperone network we have exploited high-throughput approaches to identify genetic and physical interactors of the yeast p23 cochaperone Sba1, which serves one branch of the yeast Hsp90 (Hsp82) chaperone system. Unexpectedly, bioinformatic analysis indicates that the Sba1 subnet both overlaps with and is sovereign to the identified Hsp82 network. Notably, a significant share of the Sba1 hits function at or near DNA. Thus, Sba1 appears to extend the role of the Hsp82 system to a variety of nuclear events including transcription, chromatin, telomere and DNA repair pathways. We will focus on telomere and DNA repair paths to better understand how component ts of the Hsp90 system affect nuclear events. To investigate the influence of the Sba1 chaperone we will follow a three-pronged strategy in which a) in vivo assays will be utilized to establish the physiological effect of Sba1 on DNA-related activities; b) genetic phenotypes will be employed to identify functional sba1 alleles; c) the molecular mechanisms of chaperone-regulation on client proteins will be delineated using in vitro assays. Our studies will contribute to the comprehension of how the Hsp90 chaperone network maintains cellular proteostasis and how this broad chaperone system might be dissected by targeting cochaperones to develop more selective therapeutic strategies that are directed at the Hsp90 machinery.
描述(由申请人提供):我们的长期目标是了解Hsp90分子伴侣系统的细胞广度和影响。为了剖析这个庞大而多样的伴侣网络,我们利用高通量方法来确定酵母p23辅伴侣Sba1的遗传和物理相互作用,它服务于酵母Hsp90(Hsp82)伴侣系统的一个分支。出乎意料的是,生物信息学分析表明,Sba1子网既与已确定的Hsp82网络重叠,也是其独占网络。值得注意的是,相当大比例的Sba1 His在DNA上或附近发挥作用。因此,Sba1似乎将HSP82系统的作用扩展到各种核事件,包括转录、染色质、端粒和DNA修复途径。我们将重点研究端粒和DNA修复途径,以更好地了解Hsp90系统的组成部分ts如何影响核事件。为了研究Sba1伴侣的影响,我们将遵循一个三管齐下的策略:a)利用体内试验来确定Sba1对DNA相关活动的生理作用;b)利用遗传表型来鉴定功能上的sba1等位基因;c)利用体外试验来描述伴侣调节客户蛋白的分子机制。我们的研究将有助于理解Hsp90伴侣网络如何维持细胞蛋白稳定,以及如何通过靶向辅伴侣来解剖这个广泛的伴侣系统,以开发针对Hsp90机制的更具选择性的治疗策略。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Brian C Freeman其他文献

Brian C Freeman的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Brian C Freeman', 18)}}的其他基金

Regulation of the Native Protein Landscape in the Nucleus by Molecular Chaperones
分子伴侣对细胞核中天然蛋白质景观的调节
  • 批准号:
    10641829
  • 财政年份:
    2020
  • 资助金额:
    $ 29.13万
  • 项目类别:
Regulation of the Native Protein Landscape in the Nucleus by Molecular Chaperones
分子伴侣对细胞核中天然蛋白质景观的调节
  • 批准号:
    10244899
  • 财政年份:
    2020
  • 资助金额:
    $ 29.13万
  • 项目类别:
Regulation of the Native Protein Landscape in the Nucleus by Molecular Chaperones
分子伴侣对细胞核中天然蛋白质景观的调节
  • 批准号:
    10437868
  • 财政年份:
    2020
  • 资助金额:
    $ 29.13万
  • 项目类别:
2019 Stress Proteins in Growth, Development, and Disease GRC/GRS
2019 生长、发育和疾病 GRC/GRS 中的应激蛋白
  • 批准号:
    9762269
  • 财政年份:
    2019
  • 资助金额:
    $ 29.13万
  • 项目类别:
The Hsp90 molecular chaperone system
Hsp90分子伴侣系统
  • 批准号:
    8660046
  • 财政年份:
    2011
  • 资助金额:
    $ 29.13万
  • 项目类别:
The Hsp90 molecular chaperone system
Hsp90分子伴侣系统
  • 批准号:
    8300088
  • 财政年份:
    2011
  • 资助金额:
    $ 29.13万
  • 项目类别:
The Hsp90 molecular chaperone system
Hsp90分子伴侣系统
  • 批准号:
    9256500
  • 财政年份:
    2011
  • 资助金额:
    $ 29.13万
  • 项目类别:
The Hsp90 molecular chaperone system
Hsp90分子伴侣系统
  • 批准号:
    8186164
  • 财政年份:
    2011
  • 资助金额:
    $ 29.13万
  • 项目类别:
Dynamic signaling by intracellular hormone receptors
细胞内激素受体的动态信号传导
  • 批准号:
    8009196
  • 财政年份:
    2010
  • 资助金额:
    $ 29.13万
  • 项目类别:
Dynamic signaling by intracellular hormone receptors
细胞内激素受体的动态信号传导
  • 批准号:
    7368614
  • 财政年份:
    2006
  • 资助金额:
    $ 29.13万
  • 项目类别:

相似海外基金

Determining 4-Dimensional Foot Loading Profiles of Healthy Adults across Activities of Daily Living
确定健康成年人日常生活活动的 4 维足部负荷曲线
  • 批准号:
    2473795
  • 财政年份:
    2024
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Studentship
Developing a trunk function assessment for hemiplegics. -For improving activities of daily living-
开发偏瘫患者的躯干功能评估。
  • 批准号:
    23K10540
  • 财政年份:
    2023
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Relation with the activities of daily living and the subjective values among people with social withdrawal
社交退缩者日常生活活动与主观价值观的关系
  • 批准号:
    23K16596
  • 财政年份:
    2023
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
CRII: RI: Understanding Activities of Daily Living in Indoor Scenarios
CRII:RI:了解室内场景中的日常生活活动
  • 批准号:
    2245652
  • 财政年份:
    2023
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Standard Grant
Sources of vulnerability among those using homecare despite having no limitations in Activities of Daily Living. An intersectionality analysis
尽管日常生活活动没有限制,但使用家庭护理的人的脆弱性来源。
  • 批准号:
    499112
  • 财政年份:
    2023
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Operating Grants
Association between Nursing Care and Prognosis and Activities of Daily Living in Acute Stroke patients by using Big Data.
利用大数据研究急性脑卒中患者的护理与预后和日常生活活动的关系。
  • 批准号:
    23K16412
  • 财政年份:
    2023
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Effects of a model of nurses-occupational therapists collaborative practice on activities of daily living in elderly patients
护士-职业治疗师合作实践模式对老年患者日常生活活动的影响
  • 批准号:
    22K17540
  • 财政年份:
    2022
  • 资助金额:
    $ 29.13万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Synergizing home health rehabilitation therapy to optimize patients’ activities of daily living
协同家庭健康康复治疗,优化患者的日常生活活动
  • 批准号:
    10429480
  • 财政年份:
    2022
  • 资助金额:
    $ 29.13万
  • 项目类别:
Assessing a Novel Virtual Environment that Primes Individuals Living with AD/ADRD to Accomplish Activities of Daily Living.
评估一种新颖的虚拟环境,该环境可以帮助 AD/ADRD 患者完成日常生活活动。
  • 批准号:
    10668160
  • 财政年份:
    2022
  • 资助金额:
    $ 29.13万
  • 项目类别:
Synergizing home health rehabilitation therapy to optimize patients’ activities of daily living
协同家庭健康康复治疗,优化患者的日常生活活动
  • 批准号:
    10621820
  • 财政年份:
    2022
  • 资助金额:
    $ 29.13万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了