Developing a spectroscopic toolkit for probing protein structure and folding

开发用于探测蛋白质结构和折叠的光谱工具包

• 批准号: 8452775
• 负责人: Carlos Raul Baiz
• 金额: $ 1.85万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-15 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8452775
• 关键词: Address; Alzheimer' s Disease; Amides; Biological Models; Biological Process; Biophysics; Cereals; DNA Sequence Rearrangement; Data; Development; Drug Interactions; Environment; Event; Free Energy; Frequencies; Genetic; Goals; Heterogeneity; Huntington Disease; Hydrogen Bonding; Kinetics; Lasers; Lead; Link; Maps; Measures; Methods; Modeling; Molecular; Molecular Structure; Motion; Neurodegenerative Disorders; Parkinson Disease; Pathway interactions; Process; Protein Denaturation; Proteins; Resolution; Sampling; Side; Simulate; Solutions; Solvents; Spectrum Analysis; Speed; Stretching; Structure; Techniques; Theoretical Studies; Thermodynamics; Time; Vertebral column; aggregation pathway; base; cluster computing; flexibility; globular protein; infrared spectroscopy; millisecond; molecular dynamics; nanosecond; network models; protein aggregation; protein folding; protein structure; public health relevance; research study; response; simulation; temperature jump; tool; two-dimensional; vibration

DESCRIPTION (provided by applicant): Understanding protein folding from a molecular perspective is one of the central challenges in modern biophysics. Current experimental techniques only provide a relatively limited view of the ultrafast fluctuations and long-range conformational changes that biomolecules undergo in solution. The first objective of this project is to develop an experimental framework based on ultrafast multidimensional infrared spectroscopy that will measure protein structure with sub- picosecond time resolution, offering a detailed view of non-equilibrium processes such as folding, denaturation, and protein aggregation. The experimental data will be supported by cutting-edge molecular dynamics simulations, which are now possible due to recent developments in distributed computing, as well as structure-frequency maps that provide the connection between molecular structures and vibrational frequencies. Another component of the project will aim at developing a general experimental method to quantify the structure of proteins in solution from an analytical perspective with the goal of implementing methods for measuring the structure, conformational flexibility, and solvent exposure of globular proteins.

描述（由申请人提供）：从分子角度理解蛋白质折叠是现代生物物理学的核心挑战之一。目前的实验技术只提供了一个相对有限的视图的超快波动和长程构象变化，生物分子在溶液中经历。该项目的第一个目标是开发一个基于超快多维红外光谱的实验框架，该框架将以亚皮秒时间分辨率测量蛋白质结构，提供非平衡过程的详细视图，如折叠，变性和蛋白质聚集。实验数据将得到尖端分子动力学模拟的支持，由于分布式计算的最新发展，以及提供分子结构和振动频率之间联系的结构-频率图，现在已经成为可能。该项目的另一个组成部分将旨在开发一种通用的实验方法，从分析的角度量化溶液中蛋白质的结构，目标是实施测量球状蛋白质的结构，构象灵活性和溶剂暴露的方法。