Structural requirements for the nuclear export of HIV RNA

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DESCRIPTION (provided by applicant): Eukaryotic cells control the export of RNA from the nucleus to ensure that properly processed RNAs enter the cytoplasm for gene expression. Regulated RNA export restricts the lifecycle of the pathogen human immunodeficiency virus (HIV) because the host cell prevents export of the unspliced, viral RNA genome that encodes proteins for and packages into new virions. The viral protein Rev circumvents this inhibition by oligomerizing on an intronic RNA structure called the Rev Response Element (RRE) and by recruiting the host nuclear export adaptors Crm1 and Ran. To export the RRE, the ternary complex formed between these components must favorably interact with the nuclear pore complex (NPC) to compensate for the large size and polyanionic charge that are physical barriers for the translocation of any large ribonuceloprotein complex (RNPs) through the NPC. We do not yet clearly understand how export adaptors facilitate the transport of large cargoes, like the Rev-RRE complex, since structures of NPC substrates are limited to small cargoes with single adaptors while functional evidence suggests that multiple export receptors act in concert to transport large RNPs. Likewise, we hypothesize the Rev-RRE complex organizes multiple Crm1 adaptors to facilitate export. To investigate how the ternary HIV export complex translocates through the NPC, we propose reconstituting the quaternary complex, determining its structure by cryo-electron microscopy, and using the structure to guide biochemical and cellular assays to understand how the number and position of adaptors affects export. Together these experiments will provide the physical foundation for further interrogating how the NPC coordinates multiple stages of RNA export.