Functional integration of inhibitory interneuron progenitors in the adult brain

成人大脑中抑制性中间神经元祖细胞的功能整合

• 批准号: 8452226
• 负责人: Robert F Hunt
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8452226
• 关键词: Accounting; Acute; Adult; Affect; Alzheimer' s Disease; Animals; Autistic Disorder; Brain; Brain Diseases; Brain region; Cell Therapy; Cell Transplantation; Cells; Characteristics; Cholecystokinin; Confocal Microscopy; Craniocerebral Trauma; Data; Development; Electrophysiology (science); Embryo; Epilepsy; Fellowship; Goals; Hippocampus (Brain); Human; Image; Interneurons; Laboratories; Learning; Medial; Memory; Mental disorders; Methods; Modeling; Molecular; Mood Disorders; Morphology; Mus; Neonatal; Nerve Degeneration; Neurobiology; Neurons; Parvalbumins; Pattern; Phenotype; Physiological; Population; Property; Replacement Therapy; Research; Reverse Transcriptase Polymerase Chain Reaction; Rodent; Role; Schizophrenia; Scientist; Seizures; Series; Slice; Somatostatin; Stem cells; Synapses; Techniques; Telencephalon; Therapeutic; Transplantation; Vasoactive Intestinal Peptide; biocytin; calretinin; cell motility; clinical application; dentate gyrus; design; gamma-Aminobutyric Acid; injured; interest; migration; nerve stem cell; nervous system disorder; neural circuit; patch clamp; postnatal; postsynaptic; progenitor; programs; research study; synaptic inhibition; way finding

DESCRIPTION (provided by applicant): Inhibitory interneurons perform important roles in regulating cortical network function, and their loss is associated with a number of neurological and psychiatric disorders. As such, transplantation of inhibitory neuronal progenitor cells might be a useful method to restore deficits in inhibition in the diseased brain. Our laboratory recently demonstrated that progenitor cells derived from the medial ganglionic eminence can migrate, differentiate into GABAergic interneurons, and suppress spontaneous seizures in a developmental epilepsy model. Despite these recent advances in transplantation strategies, the effect of inhibitory neural progenitor cell subtypes derived from other regions of the embryonic telencephalon on existing host circuitry remains largely unknown, especially after transplant into the adult brain. In this postdoctoral fellowship proposal, I will determine how GABA-progenitor cells derived from the caudal ganglionic eminence alter inhibitory circuitry after transplant into the adult dentate gyrus. Two specific aims are proposed, to determine: (1) progenitor cell migration and phenotypes of GABAergic subtypes generated after transplant into the adult hippocampus, and (2) how these new interneurons alter existing synaptic circuitry in the dentate gyrus. Electrophysiological results will be correlated with cellular, molecular, and anatomical characteristics. This study will provide detailed information about the synaptic connectivity of GABAergic progenitor cells after transplant into the adult brain.

描述（由申请人提供）：抑制性中间神经元在调节皮质网络功能中发挥重要作用，它们的丧失与许多神经和精神疾病有关。因此，移植抑制性神经元祖细胞可能是一种有效的方法来恢复病变大脑的抑制缺陷。我们的实验室最近