Early Detection of Evaporative Dry Eye Syndrome (EDE)

蒸发性干眼症 (EDE) 的早期检测

• 批准号: 8453089
• 负责人: Bruce Bruce Chase
• 金额: $ 13.92万
• 依托单位: PAIR TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8453089
• 关键词: Address; Affect; Age; Attenuated; Biological Assay; Chemicals; Clinical; Cornea; Corneal Ulcer; Couples; Deposition; Detection; Diagnosis; Drops; Dry Eye Syndromes; Duct (organ) structure; Dyes; Early Diagnosis; Economics; Elements; Environment; Eye; Eye diseases; Facilities and Administrative Costs; Film; Gland; Goals; Health Care Costs; Healthcare; Heating; Laboratories; Lipids; Liquid substance; Long-Term Effects; Measurement; Measures; Methods; Office Visits; Ophthalmic examination and evaluation; Ophthalmologist; Optometrist; Osmolar Concentration; Patients; Pharmaceutical Preparations; Phase; Productivity; Proteins; Research; Sampling; Small Business Innovation Research Grant; Sodium Chloride; Staining method; Stains; Surface; System; Techniques; Technology; Testing; Time; Vision; absorption; analytical method; base; commercial application; design and construction; evaporation; eye dryness; improved; innovation; instrument; instrumentation; migration; miniaturize; ocular surface; point of care; premature; programs; public health relevance; rapid diagnosis; technological innovation; user-friendly

DESCRIPTION (provided by applicant): Dry eye (DE) affects 25-30 million people in the U.S. alone and is responsible for $3.84 billion in direct annual health care costs. Evaporative dry eye (EDE) results from a lipid deficiency in tears causing the premature evaporation of tears. The problem to be solved is that current methods for accurately diagnosing EDE take time and are expensive. A fast, objective, analytical method that can be administered during a routine eye examination (10 minutes) is needed. We propose to address this need using a ultra-fast infrared spectrograph to measure the ratio of lipid to protein in tear fluid sampled with a conventional Schirmer strip; a test routinely administered during an eye exam. The chemical constituents deposited on the Schirmer strip will be measured using a unique measurement approach, which couples separation of the chemical components along the strip and measurement of the distribution of these components with a new, multichannel measurement system employing an attenuated total reflection accessory. The primary cause of EDE is rapid tear evaporation due to low lipid levels. The onset of dry eye conditions can be pre-anticipated by changes in the lipid content of tears (a decrease in lipid to protein ratio (L/P)) measured by an infrared analysis of a tear film obtained on a Schirmer test strip. The long term goal is to provide an instrument compatible with a doctor's office that can rapidly and reliably measure the lipid to protein ratio n tears. This Phase I program will determine the L/P in synthetic and natural tears sampled with a Schirmer strip in less than two minutes with sensitivity (precision) of 1%. The Phase II program will design and construct a bench-top instrument, suitable for use in an ophthalmologist's office. It will use a standard Schirmer strip containing tears extracted from a patient during a routine ophthalmic exam and provide a measure of the lipid to protein ratio. There are more than 18,000 practicing ophthalmologists in the U.S. today. This "user-friendly", technician-operated, PA-IR instrument will allow them to track the L/P ratio for patients and provide for early detectio of the onset of evaporative dry eye (EDE).

描述（由申请人提供）：干眼（DE）仅在美国就影响2500万至3000万人，每年直接医疗保健费用为38.4亿美元。蒸发性干眼（EDE）是由于泪液中的脂质缺乏导致泪液过早蒸发。要解决的问题是，目前用于准确诊断EDE的方法耗时且昂贵。需要一种快速、客观的分析方法，可以在常规眼科检查（10分钟）期间进行。我们建议使用超快速红外光谱仪来解决这一需求，以测量用传统的Schirmer条带采样的泪液中脂质与蛋白质的比率;眼科检查期间常规进行的测试。沉积在Schirmer条上的化学成分将使用独特的测量方法进行测量，其将化学成分沿着带的分离和这些成分的分布的测量与新的，使用衰减全反射附件的多通道测量系统。EDE的主要原因是由于低脂质水平导致的快速泪液蒸发。干眼症的发作可以通过泪液的脂质含量的变化（脂质与蛋白质比率（L/P）的降低）来预先预测，所述变化通过对泪液的红外分析来测量。 在Schirmer测试条上获得泪膜。长期目标是提供一种与医生办公室兼容的仪器，其可以快速可靠地测量泪液中的脂质与蛋白质比率。该第一阶段计划将在不到两分钟内用Schirmer条测定合成和天然泪液中的L/P，灵敏度（精度）为1%。第二阶段计划将设计和建造一个台式仪器，适用于眼科医生的办公室。它将使用标准的Schirmer试纸，其中含有在常规眼科检查期间从患者中提取的泪液，并提供脂质与蛋白质比率的测量。今天，美国有超过18，000名执业眼科医生。这种“用户友好”的技术人员操作的PA-IR仪器将允许他们跟踪患者的L/P比率，并提供蒸发性干眼（EDE）发病的早期检测。