Effects of Isolation Rearing on Dopamine Release and Reuptake

隔离饲养对多巴胺释放和再摄取的影响

• 批准号: 8508137
• 负责人: Jordan Thomas Yorgason
• 金额: $ 0.79万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2013-10-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8508137
• 关键词: Acute; Admission activity; Affect; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcoholism; Alcohols; Amygdaloid structure; Animal Housing; Animal Model; Animals; Anxiety; Area; Autoreceptors; Basic Science; Behavior; Behavioral; Brain; Chronic; Data Set; Development; Disease; Dopamine; Drug Targeting; Drug abuse; Elderly; Ethanol; Event; Exhibits; Extinction (Psychology); Housing; Human; Impulsivity; Kinetics; Lead; Life; Measures; Modeling; Monitor; National Research Service Awards; Neurons; Nucleus Accumbens; Pharmaceutical Preparations; Plastics; Play; Predisposition; Presynaptic Terminals; Property; Psychological reinforcement; Public Health; Rattus; Recurrent disease; Regulation; Relative (related person); Reporting; Research; Resistance; Resolution; Risk Factors; Role; Safety; Scanning; Signal Transduction; Slice; Social isolation; Structure; Techniques; Time; United States; United States Substance Abuse and Mental Health Services Administration; addiction; alcohol effect; alcohol reinforcement; alcohol response; alcohol seeking behavior; clinically relevant; controlled release; dopamine system; dopamine transporter; dopaminergic neuron; drinking; drug of abuse; drug reward; environmental change; environmental intervention; innovation; insight; mesolimbic system; neurochemistry; neurotransmission; presynaptic; psychologic; psychostimulant; relating to nervous system; research study; response; reuptake; social; social group; tool; transmission process; uptake

DESCRIPTION (provided by applicant): Postweanling social isolation rearing produces long term changes in many behaviors, including increased locomotor novelty response, increased anxiety-like behavior, and increased ethanol consumption. In addition, isolates also show changes in dopaminergic activity, including increases in ventral tegmental dopamine neuron burst firing, increased accumbal dopamine, and increased dopamine turnover in the accumbens and amygdala. However, these previous studies are limited in that they do not reveal aspects of presynaptic terminal function. Fast scan cyclic voltammetry is a powerful tool for measuring presynaptic function due to its high temporal and spatial resolution. In the present study, we will investigate uptake rates and autoreceptor sensitivity as well as ethanol responsiveness in social isolation and group housing reared animals using voltammetry. We will be focusing on function within the nucleus accumbens and basolateral amygdala, as these areas believed to be important for ethanol's reinforcing properties.

描述(由申请者提供)：断奶后的社交隔离饲养会在许多行为方面产生长期的变化，包括运动新奇反应增加，焦虑样行为增加，以及酒精摄入量增加。此外，分离株还表现出多巴胺能活性的变化，包括腹侧被盖多巴胺神经元爆发式放电增加，伏隔多巴胺增加，伏隔和杏仁核多巴胺周转增加。然而，这些先前的研究是有限的，因为它们没有揭示突触前终末功能的某些方面。快速扫描循环伏安法具有较高的时间和空间分辨率，是检测突触前功能的有力工具。在这项研究中，我们将使用伏安法研究社会隔离和集体饲养动物的摄取率和自身受体敏感性以及乙醇反应。我们将专注于伏隔核和杏仁基底外侧核的功能，因为这些区域被认为对乙醇的增强特性很重要。