Slip-flow chromatography

滑流色谱法

• 批准号: 8524926
• 负责人: MARY J. WIRTH
• 金额: $ 10万
• 依托单位: BIOVIDRIA, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8524926
• 关键词: Blood capillaries; Bovine Serum Albumin; Caliber; Chromatography; Column Chromatography; Diagnostic; Diffuse; Diffusion; Disease; Equilibrium; Legal patent; Length; Licensing; Liquid Chromatography; Marketing; Methods; Modification; Paper; Particle Size; Peptides; Phase; Preparation; Proteins; Research Personnel; Silicon Dioxide; Small Business Technology Transfer Research; Speed; Surface; Technology; Time; Universities; Width; base; capillary; design; improved; nano; nanofluidic; particle; pressure; public health relevance; success

DESCRIPTION: The product will be a packed capillary for nanoUHPLC of peptides that provides a dramatic increase in speed and efficiency. Current commercial columns for high speed and efficiency use sub-2 mm particles. The proposed technology uses sub-0.2 mm particles. The ability to use such small particles for UHPLC is enabled by the phenomenon of slip flow, which gives enhanced volume flow rates in reversed-phase liquid chromatography. Slip flow is a breakthrough that overcomes the fundamental limit to peak broadening caused by the parabolic velocity profile in the mobile phase. Using nonporous sub-0.2 mm particles also eliminates intraparticle diffusion while giving high phase ratios. Preliminary results show that sub micrometer nonporous silica particles reach the diffusion limit for efficiency in protein separations, and the diffusion limit is expected to be reached for the faster diffusing peptides. This gives plate numbers that are independent of length, allowing columns less than 2 cm in length to give 100,000 plates with high speed. The specific aims are to 1) optimize the particle diameter, 2) optimize the separation length, and 3) evaluate the nano LC-MS of a tryptic digest.

描述：该产品将是一种填充毛细管，用于多肽的纳米超高效液相色谱分离，大大提高了速度和效率。目前用于高速和高效的商用色谱柱使用的是小于2毫米的颗粒。建议的技术使用小于0.2毫米的颗粒。滑流现象使超高效液相能够使用如此小的颗粒，从而提高了反相液相色谱中的体积流速。滑移流是一项突破，它克服了流动相中抛物线速度分布对峰展宽的基本限制。使用无孔的小于0.2 mm的颗粒还可以消除颗粒内扩散，同时提供高相比。初步结果表明，亚微米级无孔二氧化硅颗粒在蛋白质分离中达到扩散极限，扩散速度较快的多肽有望达到扩散极限。这给出了与长度无关的板号，允许长度小于2厘米的柱以高速提供100,000块板材。具体目标是1)优化颗粒直径，2)优化分离长度，3)评价胰酶消化液的纳米LC-MS。