FASEB SRC on Ciliate Molecular Biology

描述（由申请人提供）：对纤毛虫原生动物的研究导致了关于普遍生物过程的关键发现。这些包括自剪接RNA 10、端粒酶5，8.通过组蛋白乙酰转移酶的染色质重塑6.，和翻译后微管蛋白修饰13，14，15。最近在纤毛虫中的研究为非编码RNA在程序性DNA缺失和染色质重塑中的作用提供了新的见解，这是大核发育的标志11，16。纤毛虫最近被用来研究与人类疾病相关的保守细胞过程4，12。全基因组序列的可用性极大地增强了用于基因发现和新的和保守的基因的功能分析的经典和分子遗传学方法2，3，7。RNA干扰等分子工具的持续发展为研究这一进化分支所特有的或在包括人类在内的各种真核生物中保守的许多生物过程提供了更多的机会。我们一年两次的会议汇集了来自不同领域的研究人员，他们通过一个共同的主题团结在一起-使用纤毛原生动物作为实验模型系统。2013年FASEB会议将为促进未发表研究的传播提供一个重要场所。它还将作为一个场所，分享重要的纤毛虫分子生物学社区的所有成员的一般技术进步，并进一步组织社区为未来的工具和资源开发。纤毛虫在本科实验室课堂上的使用也成为我们研究界越来越感兴趣的话题。2013年会议将在科罗拉多的汽船泉举行六天。我们预计将有150- 200名参与者出席，并寻求资金来增加参与，特别是来自代表性不足背景的科学家，以及我们的下一代科学家（本科生，研究生和博士后）。

DESCRIPTION (provided by applicant): Studies on ciliated protozoa have led to key discoveries about universal biological processes. These include self-splicing RNA10, telomerase5,8., chromatin remodeling by histone acetyl-transferases6., and post-translational tubulin modifications13,14,15.. Recent studies in ciliates have provided novel insights into the role of non-coding RNAs in programmed DNA deletion and chromatin remodeling, hallmarks of macronuclear development11,16.. Ciliates have recently been exploited to study conserved cellular processes associated with human disease4,12.. The availability of full genome sequences has greatly enhanced classical and molecular genetic approaches for gene discovery and the functional analysis of novel and conserved genes2,3,7. The continued development of molecular tools, such as RNA interference, provide greater opportunities to study numerous biological processes that are unique to this evolutionary clade or are conserved in a wide variety of eukaryotic organisms, including humans. Our biannual meetings bring together investigators from various fields, united by a common theme- the use of ciliated protozoa as an experimental model system. The 2013 FASEB conference will provide an important venue to promote the dissemination of unpublished research. It will also serve as a venue to share general technical advances of importance to all members of the ciliate molecular biology community, and to further organize the community for future tool and resource development. The use of Ciliates in the undergraduate laboratory classroom has also become a topic of growing interest to our research community. The 2013 conference will be held over six days at the Steamboat Springs, Colorado, FASEB retreat location. We anticipate attendance by 150- 200 participants, and seek funds to increase participation especially by scientists from under-represented backgrounds, as well as by our next generation of scientists (undergraduates, graduate students and post-docs).