Evaluation of Fecal Salmonella Carriage in Symptomatic and Asymptomatic Dogs in t

项目总结：此项目的目的是确定是否 粪便沙门氏菌定植存在质的和量的差异 在太平洋西北地区有症状和无症状的狗之间 美国。沙门氏菌病是最重要的食源性疾病之一。 在世界范围内造成巨大的医疗和经济负担。虽然食物是 人类感染沙门氏菌的主要来源非食品性沙门氏菌来源 可以包括宠物(特别是狗和猫)以及它们的食物、零食和 补充剂。沙门氏菌病在人类或 动物是胃肠道疾病，沙门氏菌通过感染传播 粪便。尤其是狗，在受到感染时通常不会表现出沙门氏菌病的迹象。 最近的研究表明，14%到25%的无症状家犬可以 将沙门氏菌排放到它们的环境中。还有最近爆发的人类 沙门氏菌病，主要发生在蹒跚学步的儿童中，与商业干狗粮有关。 在与以下因素相关的知识方面仍然存在很大差距 有胃肠道和无胃肠道犬携带沙门氏菌的差异 疾病。这项提议将检验这样一个假设，即携带沙门氏菌的狗- 相关的胃肠道疾病有更高的粪便患病率 沙门氏菌的定植比无症状的狗，以及更高的粪便数量 沙门氏菌细菌比无症状狗的粪便沙门氏菌。粪便和 宠物相关管理数据至少来自150个症状性数据和150个 无症状的狗将从送往兽医的狗中收集 华盛顿州立大学兽医学院教学医院 以及来自阿拉斯加、爱达荷州、蒙大拿州、俄勒冈州和华盛顿州的私人兽医。 将使用ISO的标准化方法培养粪便中的沙门氏菌 标准6579：2002识别到特定的血清型，并使用 传统的最可能数(MPN)培养技术。评选结果 研究应提供有关糖尿病患病率和危险因素的新信息 沙门氏菌在无症状和有症状犬中的定植 兽医临床，以及细菌负荷与胃肠道的关系 疾病。

Project Summary: The purpose of this project is to determine whether or not there are qualitative and quantitative differences in fecal Salmonella colonization between symptomatic and asymptomatic dogs in the Pacific Northwest region of the USA. Salmonellosis is one of the most important food-borne diseases and causes substantial medical and economic burden worldwide. Although food is the main source of Salmonella infection in humans, non-food Salmonella sources can include pets (particularly dogs and cats), as well as their foods, snacks, and supplements. The most common symptoms of salmonellosis in humans or animals is gastrointestinal disease and Salmonella bacteria are shed via infected feces. Dogs in particular often do not show signs of salmonellosis when infected. Recent studies show that 14% to 25% of asymptomatic household dogs can shed Salmonella into their environment. Also a recent outbreak of human salmonellosis, primarily in toddlers, was linked to commercial dry dog food. There remains a significant gap in knowledge regarding factors associated with differences in Salmonella carriage between dogs with or without gastrointestinal disease. This proposal will test the hypothesis that dogs with Salmonella- associated gastrointestinal disease have both a higher prevalence of fecal Salmonella colonization than asymptomatic dogs, and higher fecal counts of Salmonella bacteria than asymptomatic dogs with fecal Salmonella. Feces and pet-related management data from a minimum of 150 symptomatic and 150 asymptomatic dogs will be collected from dogs presented to the veterinary teaching hospital at Washington State University, College of Veterinary Medicine and private veterinarians from Alaska, Idaho, Montana, Oregon, and Washington. Feces will be cultured for Salmonella using standardized methods from ISO standard 6579:2002 identified to specific serotype, and quantified using conventional most-probable-number (MPN) culture techniques. Results of the study should provide new information regarding prevalence and risk factors for Salmonella colonization in asymptomatic and symptomatic dogs brought to veterinary clinics, and the relationship of bacterial load to gastrointestinal disease.