Histology and Imaging Core

组织学和成像核心支持分析突变耳和固定组织中所有水平的连接缺陷。该核心的目标是提供必要的培训和某些服务，以在所有光和电子显微镜水平上处理和成像耳朵，包括使用荧光标记和3D重建的高分辨率多光子成像，以加速对现有和即将分析的突变体或其他操纵耳朵的分析，包括所需的人耳样本。为此，核心通过核心人员提供专业知识，包括在所有发育阶段评估耳朵和神经元连接所需的所有技术方面受过专业培训的全职RA。该核心的设备（三个共聚焦系统、高分辨率透射和落射荧光显微镜和解剖镜、TEM和SEM、冷冻切片机、振动切片机和超微切片机）的预订将在线处理。组织学和影像学核心的具体目标是：（1）提供一系列服务（建议和/或协助）在所有水平的光学和电子显微镜下进行优化和标准化的组织学处理，包括复杂的荧光免疫细胞化学标记，然后进行多光子分析，或原位杂交和免疫细胞化学的组合;（2）通过提供和使用适当的图像处理和定量分析软件，改进数据分析;（3）提供这些技术方面的专家培训，以促进核心赠款成员与目前通过NIDCD供资的潜在额外成员之间的合作。组织学核心与其他核心（如分子核心）密切相互作用，以生成原位探针。核心将由几个研究人员的需求驱动，以实现对通过遗传或其他操作产生的各种小鼠模型的标准化高分辨率分析。核心人员的科学专门知识将提高所有核心相关研究的绩效。一年一度的研讨会，提供了在这个核心可用的技术和他们的潜在有用性的研究核心的当前和未来的成员的概述将产生一个至关重要的互动对听力研究相关的社区在爱荷华州的大学。

The Histology and Imaging core supports analysis of mutant ears and connectional defects at all levels in fixed tissue. The goal of this core is to provide the necessary training and certain services to process and image ears at all light and electron microscopic levels, including high resolution multiphoton imaging using multicolor labeling and 3D reconstruction to accelerate analysis of existing and soon to be analyzed mutants or otherwise manipulated ears, including samples of human ears as needed. To this end, the core provides expertise through the core personnel, including a full time RA expertly trained in all techniques needed to evaluate ears and neuronal connections at all stages of development. Reservation of the equipment in this core (three confocal systems, high resolution transmitted and epifluorescence microscopes and dissecting scopes, TEM and SEM, freezing microtomes, vibratomes and ultramicrotomes) will be handled on line. The specific aims of the histology and imaging core are: (1) To provide a range of service (advice and/or assistance) for optimized and standardized histological processing at all levels of light and electron microscopy, including sophisticated multicolor immunocytochemical labeling followed by multiphoton analysis, or combinations of in situ hybridization and immunocytochemistry; (2) To improve data analysis through the availability and use of appropriate image processing and quantitative analysis software; (3) To provide expert training in these techniques to stimulate collaboration between members of the core grant as well as potential additional members currently funded through NIDCD. The histology core closely interacts with other cores such as the molecular core for the generation of in situ probes. The core will be driven by the needs of several researchers to achieve a standardized high resolution analysis of various mouse models generated by genetic or other manipulations. The scientific expertise of the core personnel will enhance the performance of all core related research. An annual workshop that provides overviews of the techniques available in this core and their potential usefulness for research of current and future members of the core will generate a vital interaction toward a hearing research related community at the Univ. of Iowa.