Regulation of germline stem cell aging by insulin S.ignaling

描述(由申请人提供)：干细胞不能随着年龄的增长而保持，与许多生物体，包括人类的组织退化和对组织损伤的敏感性增加等情况有关。这项提案以线虫生殖系为模型，阐述了衰老如何影响干细胞。利用线虫及其生殖系的研究揭示了衰老和干细胞生物学的保守机制，因此该建议结合了成熟的衰老研究遗传模型和定义明确、可访问的干细胞系统，为剖析衰老对干细胞动力学的影响提供了独特的机会。我们将确定干细胞耗竭的细胞机制以及通过胰岛素/胰岛素样生长因子信号通路预防干细胞耗竭。我们还将确定胰岛素/胰岛素样生长因子信号通路这种特殊的抗衰老作用所需的特定组织。这些Pilo研究将为今后的工作奠定基础。

DESCRIPTION (provided by applicant): Failure to maintain stem cells with age is associated with conditions such as tissue degeneration and increased susceptibility to tissue damage in many organisms, including humans. This proposal addresses how aging affects stem cells using the C. elegans germ line as a model. Studies using C. elegans and its germ line have revealed conserved mechanisms of aging and stem cell biology, therefore this proposal combines a well-established genetic model for aging studies and a well- defined, accessible stem cell system, providing a unique opportunity to dissect the effects of aging on stem cell dynamics. We will determine the cellular mechanisms underlying stem cell depletion and its prevention by the insulin/IGF-signaling pathway. We will also determine the specific tissue requirements for this particular anti-aging effect of the insulin/IGF-signaling pathway. These pilo studies will lay the groundwork for future work.