CELL MORHOLOGY CORE

细胞形态学核心

• 批准号: 8502520
• 负责人: Peter L Bell
• 金额: $ 10.09万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8502520
• 关键词: Area; Arterial Fatty Streak; Blood Vessels; Cardiovascular Diseases; Cells; Cellular Morphology; DNA Viruses; Electron Microscope; Electron Microscopy; Equipment; Freezing; Gene Expression; Grant; Heart; Histology; Histopathology; Housing; Immunohistochemistry; In Situ Hybridization; Laboratories; Liver; Microscopy; Paraffin; Pathologic; Pennsylvania; Preparation; Reporter Genes; Sampling; Specimen; Staining method; Stains; Supporting Cell; Technology; Tissues; Toxic effect; Transgenes; Universities; Work; fluorescence microscope; gene therapy; programs; square foot; therapeutic transgene; tissue preparation; tissue processing; transgene expression; vector

The Cell Morphology Core is an independent and fully equipped laboratory capable of conducting a wide array of studies necessary to characterize tissue and cell morphology, as well as localize transgenes and transgene products. This Core is directed by Dr. Peter Bell and is housed in 800 square feet of dedicated space and includes two fluorescent microscopes, one electron microscope, and all the equipment necessary to process tissues and cells for a wide array of histological analysis. The Core will provide extensive support to all three of the projects in the area of 1) preparation of tissues for histopathologic analysis, 2) special stains for more detailed characterization of pathologic specimens, 3) analysis of tissues for transgene expression using immunqhistochemistry and in situ hybridization; 4) quantitative analysis of reporter gene expression in whole liver and liver sections using the Xenogen technology; and 5) EM analysis of vector preps.

细胞形态学核心是一个独立的，设备齐全的实验室，能够进行广泛的 一系列必要的研究来表征组织和细胞形态，以及定位转基因和 转基因产品这个核心是由彼得贝尔博士指导，并设在800平方英尺的专用 空间，包括两个荧光显微镜，一个电子显微镜，以及所有必要的设备 来处理组织和细胞以进行广泛的组织学分析。核心将提供广泛的支持 所有三个项目领域的1）准备组织病理学分析，2）特别 染色以更详细地表征病理标本，3）分析组织中的转基因 免疫组化和原位杂交检测报告基因的表达; 4）报告基因的定量分析 使用Xenogen技术在全肝和肝切片中表达;和5）载体的EM分析 准备