MicroRNAs in Kidney Progenitor Cells.
肾祖细胞中的 MicroRNA。
基本信息
- 批准号:8531232
- 负责人:
- 金额:$ 23.51万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-09-01 至 2015-07-31
- 项目状态:已结题
- 来源:
- 关键词:AlgorithmsApoptosisBioinformaticsBiological ModelsBostonCell physiologyCellsChildChildhoodComplementDataDevelopmental ProcessEmbryoEndowmentEquilibriumFoundationsFunctional disorderGene ExpressionGrantHealthIndividualJordanKidneyKidney FailureKnowledgeMentorsMessenger RNAMicroRNAsModelingMolecularNephronsOrgan Culture TechniquesPediatric HospitalsPhasePhenotypePhysiciansPlayPopulationResearchRoleScientistSmall RNAStem cellsTestingTranscriptTransgenic MiceTransgenic OrganismsWorkabstractingbody systemcareerembryonic stem cellgain of functiongene repressionimprovedinterestloss of functionmedical schoolsmembermouse modelnephrogenesisnovelpeptide analogprematurepro-apoptotic proteinprogenitorself-renewalsmall hairpin RNA
项目摘要
Project Summary/Abstract
MicroRNAs (miRNAs) are a group of novel small RNAs that regulate gene expression via the post-
transcriptional repression of specific target mRNAs. As a group, miRNAs play a key role in diverse
developmental processes, and are required for the differentiation of embryonic stem cells; however the
function of miRNAs during kidney development remains largely undefined. Our preliminary work indicates that
the loss of miRNAs within the nephron progenitor compartment of the developing kidney results in a premature
depletion of this population, and as a consequence, a marked decrease in nephron number. Furthermore, this
is accompanied by elevated expression of the pro-apoptotic protein Bim (also known as Bcl-2L11) specifically
in nephron progenitors, and an increase in apoptosis in this cell population. We propose that specific miRNAs
promote the survival of nephron progenitors by regulating the expression of Bim, and that this mechanism
represents a means of determining congenital nephron endowment during normal kidney development.
Specific aim 1: To characterize the role of Bim (Bcl-2L11) in the survival of nephron progenitors during kidney
development. Specific aim 2: To define the function of the miRNA clusters, mmu-miR-106b~25 and mmu-
miR-17~92, in regulating Bim expression and nephron progenitors. Specific aim 3: To determine the
functional role of mmu-miR-10a during kidney development. The work proposed in this grant will serve as the
foundation for the PI's transition into an independent career as a physician-scientist in an academic pediatric
renal division over the next two years. The mentored phase (K99) will occur at Children's Hospital Boston and
Harvard Medical School under the guidance of Dr. Jordan Kreidberg.
项目摘要/摘要
MicroRNAs(MiRNAs)是一组新型的小RNA,通过转录后调节基因表达。
转录抑制特定靶基因的mRNAs。作为一个群体,miRNAs在不同的
发育过程,是胚胎干细胞分化所必需的;然而
MiRNAs在肾脏发育过程中的作用在很大程度上仍不清楚。我们的初步工作表明,
发育中肾脏的肾单位祖细胞体内miRNAs的丢失导致早产
这一群体的枯竭,其结果是肾单位数量显著减少。此外,这一点
伴随着促凋亡蛋白Bim(也称为Bcl-2L11)的特异性表达增加
在肾单位祖细胞中,以及在这一细胞群体中的凋亡率增加。我们建议特定的miRNAs
通过调节Bim的表达促进肾单位祖细胞的存活,这一机制
代表了一种确定正常肾脏发育过程中先天性肾单位天赋的方法。
特异目的1:研究Bim(Bcl2L11)在肾小球祖细胞存活中的作用
发展。具体目标2:确定miRNA簇MMU-miR-106b~25和MMU-miR-106b~25的功能
MIR-17~92,调节Bim表达和肾单位祖细胞。具体目标3:确定
MMU-miR-10a在肾脏发育中的功能作用这项拨款中建议的工作将作为
为PI过渡到独立的职业生涯奠定基础,成为一名学术儿科的内科科学家
在接下来的两年里,肾脏部门。指导阶段(K99)将在波士顿儿童医院和
哈佛医学院在乔丹·克莱德伯格博士的指导下。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('JACQUELINE HO', 18)}}的其他基金
Regulation of tubulointerstitial crosstalk by microRNAs in renal fibrosis
肾纤维化中 microRNA 对肾小管间质串扰的调节
- 批准号:
10749334 - 财政年份:2023
- 资助金额:
$ 23.51万 - 项目类别:
The University of Pittsburgh Summer Research Internship Program kidney workshop (SRIP-Kid)
匹兹堡大学夏季研究实习计划肾脏研讨会(SRIP-Kid)
- 批准号:
10371022 - 财政年份:2021
- 资助金额:
$ 23.51万 - 项目类别:
The University of Pittsburgh Summer Research Internship Program kidney workshop (SRIP-Kid)
匹兹堡大学夏季研究实习计划肾脏研讨会(SRIP-Kid)
- 批准号:
10623196 - 财政年份:2021
- 资助金额:
$ 23.51万 - 项目类别:
Endothelial miR-17~92 protects against acute kidney injury
内皮miR-17~92预防急性肾损伤
- 批准号:
10338136 - 财政年份:2020
- 资助金额:
$ 23.51万 - 项目类别:
Endothelial miR-17~92 protects against acute kidney injury
内皮miR-17~92预防急性肾损伤
- 批准号:
10550222 - 财政年份:2020
- 资助金额:
$ 23.51万 - 项目类别:
Endothelial miR-17~92 protects against acute kidney injury
内皮miR-17~92预防急性肾损伤
- 批准号:
10117251 - 财政年份:2020
- 资助金额:
$ 23.51万 - 项目类别:
The Role of miR-17~92 in Nephron Progenitors
miR-17~92在肾单位祖细胞中的作用
- 批准号:
9331615 - 财政年份:2014
- 资助金额:
$ 23.51万 - 项目类别:
The Role of miR-17~92 in Nephron Progenitors
miR-17~92在肾单位祖细胞中的作用
- 批准号:
8798885 - 财政年份:2014
- 资助金额:
$ 23.51万 - 项目类别:
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