Neutralizing antibodies scientific research support component

中和抗体科学研究支持组件将为CHAVI提供必要的实验室和科学支持-通过在标准化和验证的测定中对1级和2级参考HIV-I菌株进行中和活性的高通量评估。此外，我们将绘制广泛中和血清和单克隆抗体的表位，监测疫苗引起的nAb反应，以确定改进的免疫原，佐剂和载体，并使用来自2b期试验的突破性病毒将nAb描述为保护的相关物。 具体目标 目标1.通过定义选定临床组血清中中和活性的幅度和宽度（M-B）以及通过绘制广泛中和血清的表位来支持CHAVI-ID研究。 目标二。通过筛选记忆B细胞培养上清液以鉴定产生nAb的B细胞来支持CHAVI-ID研究。 目标3.通过表征新mAb的中和活性和表位特异性的大小和宽度来支持CHAVI-ID研究。 目标4。通过监测疫苗在动物、人类疫苗接种者中引发的nAb的幅度和广度，以及通过评估疫苗血清与人类疫苗突破性感染病毒的中和作用，支持CHAVI-ID研究。

The Neutralizing Antibody Scientific Research Support Component will provide essential laboratory and scientific support for CHAVI- by performing high throughput assessments of neutralizing activity against tier 1 and tier 2 reference HlV-1 strains in standardized and validated assays. In addition, we will map the epitopes of broadly neutralizing sera and mAbs, monitor vaccine-elicited nAb responses to identify improved immunogens, adjuvants and vectors, and delineate nAbs as a correlate of protection using breakthrough viruses from phase 2b trials. Specific Aims Aim 1. To support CHAVI-ID research by defining the magnitude and breadth (M-B) of neutralizing activity in sera from select clinical groups, and by mapping the epitopes of broadly neutralizing sera. Aim 2. To support CHAVI-ID research by screening memory B cell culture supernatants to identify nAb-producing B cells. Aim 3. To support CHAVI-ID research by characterizing new mAbs for magnitude and breadth of neutralizing activity and epitope specificity. Aim 4. To support CHAVI-ID research by monitoring the magnitude and breadth of vaccine-elicited nAbs in animals, human vaccinees, and by assessing vaccine sera neutralization with viruses from vaccine breakthrough infections in humans.