Imaging Core

成像核心

• 批准号: 8376990
• 负责人: Pablo A. Ortiz
• 金额: $ 18.22万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8376990
• 关键词: Antibodies; Argon; Calibration; Cells; Computer software; Computers; Confocal Microscopy; Contract Services; Cultured Cells; Custom; Data Analyses; Devices; Ensure; Equipment; Eye; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorescent Antibody Technique; Fluorescent Dyes; Goat; Half-Life; Human Resources; Image; Immersion Investigative Technique; Immunofluorescence Immunologic; Individual; International; Ionomycin; Kidney; Label; Lasers; Life; Light; Light Microscope; Maintenance; Microscope; Microscopy; Modification; Mus; Nystatin; Oils; Optics; Oryctolagus cuniculus; Paper; Perfusion; Pluronics; Productivity; Program Research Project Grants; Reagent; Research Personnel; Role; Scanning; Schedule; Services; Source; System; Techniques; Temperature; Time; Tissues; Training; Video Microscopy; Work; blood pressure regulation; cell fixing; cellular imaging; charge coupled device camera; cost; data acquisition; density; design; dihydroethidium; improved; lens; light microscopy; novel; research study; response; sodium green; sodium-binding benzofuran isophthalate; wasting

The Imaging Core (Core C) will be crucial for the quality and productivity of all participating projects. The Imaging Core centralizes the equipment, purchasing of supplies, training and scheduling required to complete the imaging experiments proposed in the four projects comprising this Program Project Grant. The microscopy imaging experiments that will be supported by this core include: light, fluorescence, laser scanning confocal and time-lapse confocal microscopy. Core C will provide investigators with the necessary microscopy equipment, which is already in place. In addition, Core C staff will provide the professional training required for specific imaging applications, including the use of hardware, specific imaging software, data acquisition and analysis necessary to carry out the proposed experiments accurately and in a timely manner. This will increase the efficiency and productivity of the Program Project Grant, because all personnel will be properly trained, and common techniques will be standardized. This will likewise reduce costs by eliminating duplication of efforts. Core C will also provide all of the reagents required for imaging, including fluorescent antibodies and dyes. This will eliminate waste and duplication of supplies and effort. Additionally, Core C will maintain all equipment through service contracts. This will ensure that all equipment is in proper working order when needed by individual projects, thereby reducing downtime and increasing efficiency.

成像核心(核心C)将对所有参与项目的质量和生产率至关重要。这个 图像核心集中了设备、用品的采购、培训和所需的日程安排 完成本计划资助的四个项目中提出的成像实验。这个 该核心支持的显微成像实验包括：光、荧光、激光 扫描共聚焦显微镜和延时共聚焦显微镜。核心C将为调查人员提供必要的 显微镜设备，这已经到位了。此外，Core C的工作人员将提供专业的 特定成像应用所需的培训，包括使用硬件、特定成像软件、 准确和及时地进行拟议实验所需的数据采集和分析 举止。这将提高计划项目赠款的效率和生产率，因为所有 人员将得到适当培训，通用技术将得到标准化。这也将同样减少 通过消除重复工作来降低成本。核心C还将提供成像所需的所有试剂， 包括荧光抗体和染料。这将消除供应和工作的浪费和重复。 此外，核心C将通过服务合同维护所有设备。这将确保所有设备 在个别项目需要时处于适当的工作状态，从而减少停机时间并增加 效率。