Acute Renal Failure: An Endotoxin Hyper-Responsive State

急性肾衰竭:内毒素高反应状态

基本信息

  • 批准号:
    8541828
  • 负责人:
  • 金额:
    $ 33.9万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-08-01 至 2015-07-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): In the aftermath of acute renal injury (AKI), proximal tubular cells manifest dramatic hyper-responsiveness to Toll receptor ligands, most notably, endotoxin. This exaggerates renal cytokine (e.g., TNF-1) / chemokine (e.g., MCP-1) production which can worsen the severity of acute renal failure (ARF). With renal venous cytokine efflux, extra-renal injury may also result. This LPS hyper-responsiveness is transcriptionally regulated, and mediated, in part, by gene activating chromatin events. The overall goal of this proposal is to: i) further define the pathways which induce the underlying chromatin changes; and ii) delineate how they mediate an exaggerated LPS responsive state. To focus the application, one ARF model (ischemia/reperfusion) 1 exposure to one Toll ligand (LPS) will be tested in three Specific Aims: Aim#1: Define the temporal sequence of chromatin changes along the TNF-1 gene in AKI. We will characterize chromatin changes, including CpG methylation/demethylation, histone acetylation, methylation, histone variants, and alterations in nucleosome positions. Both renal injury, and proximal tubule-specific injury (in cultured tubular HK-2 cells), will be studied following reversible ischemia + LPS. These in vivo and in vitro experiments will set the stage for mechanistic assessments (Aim 3). Aim #2: Ascertain which transcription and chromatin remodeling factors / enzymes are recruited to the TNF-1 gene in AKI. We have already shown that the chromatin remodeler BRG1 is recruited to the TNF-1 gene and is required for injury-induced TNF-1 transcription. We have also demonstrated that NF-:B and AP-1 transcription factors are recruited to the TNF-1 gene in AKI. Using Matrix ChIP, we will more fully define the spatiotemporal sequence of chromatin and transcription events at the TNF-1 gene in response to reversible ischemia + LPS. This will help to ascertain which factors are hyper-recruited to the TNF-1 gene and may thus drive the LPS hyper-responsive state. Aim #3 Test the mechanistic relevance of the above defined changes in the induction of the LPS hyper-responsive state. The mechanistic relevance of the above defined changes in establishing LPS hyper-responsiveness will be tested both in vitro and in vivo using siRNA targeting (as we have already done to knock-down BRG1 in HK-2 cells). We will complement these in vivo and in vitro siRNA approaches with the use of pharmacologic reagents directed at the putative molecular mediators of the hyper-responsive chromatin state. The latter approach may lead to therapeutically relevant translational approaches for modulating experimental AKI and associated multiorgan failure. The proposed studies are highly novel in that, with the exception of data obtained by the PIs, virtually no information exists as to the nature, and consequences of, chromatin alterations in response to AKI. Hence, new perspectives on mechanisms of AKI and its downstream consequences should result. PUBLIC HEALTH RELEVANCE: Acute kidney injury (AKI) leads to renal production of inflammatory mediators and sensitizes/primes the kidney to bacterial toxins. These renal inflammatory mediators gain access to the systemic circulation and can induce extra-renal tissue damage, a common cause of mortality in patients with AKI, due to infections, trauma and other illness. We propose to define the chromatin/transcriptional mechanisms for these renal responses with the goal to develop preventive treatments in the future.
描述(由申请人提供):在急性肾损伤(阿基)后,近端肾小管细胞对Toll受体配体(最显著的是内毒素)表现出显著的高反应性。这夸大了肾细胞因子(例如,TNF-1)/趋化因子(例如,单核细胞趋化蛋白-1(MCP-1)的产生,这可能会加重急性肾功能衰竭(ARF)的严重程度。随着肾静脉细胞因子流出,也可能导致肾外损伤。这种LPS高反应性是转录调节的,并且部分地由基因激活染色质事件介导。该提案的总体目标是:i)进一步定义诱导潜在染色质变化的途径;和ii)描述它们如何介导过度的LPS反应状态。为了集中应用,将在三个特定目标中测试暴露于一种Toll配体(LPS)的一种ARF模型(缺血/再灌注)1:目标#1:定义阿基中染色质变化的时间顺序沿着TNF-1基因。我们将描述染色质的变化,包括CpG甲基化/去甲基化,组蛋白乙酰化,甲基化,组蛋白变体和核小体位置的改变。将在可逆性缺血+ LPS后研究肾损伤和近端肾小管特异性损伤(在培养的肾小管HK-2细胞中)。这些体内和体外实验将为机制评估(目标3)奠定基础。 目的#2:确定哪些转录和染色质重塑因子/酶在阿基中被募集到TNF-1基因。我们已经表明,染色质重塑BRG 1被募集到TNF-1基因中,并且是损伤诱导的TNF-1转录所必需的。我们还证实了NF-:B和AP-1转录因子在阿基中被募集到TNF-1基因。使用矩阵ChIP,我们将更全面地定义在可逆性缺血+ LPS的TNF-1基因的染色质和转录事件的时空序列。这将有助于确定哪些因子被过度募集到TNF-1基因中,从而可能驱动LPS过度反应状态。 目的#3测试上述定义的变化在诱导LPS高反应状态中的机制相关性。将使用siRNA靶向在体外和体内测试上述定义的变化在建立LPS高反应性中的机制相关性(正如我们已经在HK-2细胞中敲低BRG 1所做的那样)。我们将补充这些在体内和体外siRNA的方法与使用的药理学试剂针对假定的分子介质的超反应染色质状态。后一种方法可能导致治疗相关的翻译方法,用于调节实验性阿基和相关的多器官衰竭。 拟定的研究非常新颖,因为除了PI获得的数据外,几乎没有关于阿基反应中染色质改变的性质和后果的信息。因此,阿基及其下游后果的机制的新观点应该产生。 公共卫生关系: 急性肾损伤(阿基)导致肾脏产生炎症介质,并使肾脏对细菌毒素敏感/启动。这些肾脏炎症介质进入体循环,并可诱导肾外组织损伤,这是阿基患者因感染、创伤和其他疾病而死亡的常见原因。我们建议确定这些肾脏反应的染色质/转录机制,以在未来开发预防性治疗方法。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KAROL BOMSZTYK其他文献

KAROL BOMSZTYK的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KAROL BOMSZTYK', 18)}}的其他基金

Influence of Pre-Analytical Factors in Globlastoma MGMT Promoter Methylation Biomarker Assay
预分析因素对球母细胞瘤 MGMT 启动子甲基化生物标志物测定的影响
  • 批准号:
    9975358
  • 财政年份:
    2020
  • 资助金额:
    $ 33.9万
  • 项目类别:
Influence of Pre-Analytical Factors in Globlastoma MGMT Promoter Methylation Biomarker Assay
预分析因素对球母细胞瘤 MGMT 启动子甲基化生物标志物测定的影响
  • 批准号:
    10415839
  • 财政年份:
    2020
  • 资助金额:
    $ 33.9万
  • 项目类别:
Transcriptional and epigenetic control of angiogenic genes in sepsis-induced acute kidney injury.
脓毒症引起的急性肾损伤中血管生成基因的转录和表观遗传控制。
  • 批准号:
    9173657
  • 财政年份:
    2016
  • 资助金额:
    $ 33.9万
  • 项目类别:
Transcriptional and epigenetic control of angiogenic genes in sepsis-induced acute kidney injury.
脓毒症引起的急性肾损伤中血管生成基因的转录和表观遗传控制。
  • 批准号:
    9334850
  • 财政年份:
    2016
  • 资助金额:
    $ 33.9万
  • 项目类别:
Integrated microplate platform for epigenetic analysis
用于表观遗传分析的集成微孔板平台
  • 批准号:
    8754755
  • 财政年份:
    2014
  • 资助金额:
    $ 33.9万
  • 项目类别:
Integrated microplate platform for epigenetic analysis
用于表观遗传分析的集成微孔板平台
  • 批准号:
    9066225
  • 财政年份:
    2014
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute Renal Failure: An Endotoxin Hyper-Responsive State
急性肾衰竭:内毒素高反应状态
  • 批准号:
    8118789
  • 财政年份:
    2010
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute Renal Failure: An Endotoxin Hyper-Responsive State
急性肾衰竭:内毒素高反应状态
  • 批准号:
    8305648
  • 财政年份:
    2010
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute Renal Failure: An Endotoxin Hyper-Responsive State
急性肾衰竭:内毒素高反应状态
  • 批准号:
    7982459
  • 财政年份:
    2010
  • 资助金额:
    $ 33.9万
  • 项目类别:
LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
肾小球细胞中的层粘连蛋白基因表达
  • 批准号:
    7921107
  • 财政年份:
    2009
  • 资助金额:
    $ 33.9万
  • 项目类别:

相似海外基金

Acute Kidney Failure in a Cancer ICU
癌症 ICU 中的急性肾衰竭
  • 批准号:
    7034020
  • 财政年份:
    2006
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute Kidney Failure in a Cancer ICU
癌症 ICU 中的急性肾衰竭
  • 批准号:
    7459945
  • 财政年份:
    2006
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute Kidney Failure in a Cancer ICU
癌症 ICU 中的急性肾衰竭
  • 批准号:
    7922716
  • 财政年份:
    2006
  • 资助金额:
    $ 33.9万
  • 项目类别:
Acute kidney failure: investigation and treatment of ki dney cell injury
急性肾衰竭:肾细胞损伤的调查和治疗
  • 批准号:
    nhmrc : 901011
  • 财政年份:
    1990
  • 资助金额:
    $ 33.9万
  • 项目类别:
    NHMRC Project Grants
Acute kidney failure: new methods of investigation and treatment
急性肾衰竭:研究和治疗的新方法
  • 批准号:
    nhmrc : 891081
  • 财政年份:
    1989
  • 资助金额:
    $ 33.9万
  • 项目类别:
    NHMRC Project Grants
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了