Optical imaging of the input and output of the olfactory bulb

嗅球输入和输出的光学成像

• 批准号: 8727276
• 负责人: Douglas Anthony Storace
• 金额: $ 5.51万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8727276
• 关键词: Afferent Neurons; Alzheimer' s Disease; Animals; Apical; Area; Axon; Brain; Brain region; Calcium; Cells; Complex; Dendrites; Dextrans; Disease; Dyes; Interneurons; Kinetics; Maps; Measurement; Measures; Methods; Mus; Neurons; Neuropil; Nose; Olfactory Receptor Neurons; Oregon; Output; Pattern; Preparation; Presynaptic Terminals; Process; Proteins; Retina; Sensory; Sensory Process; Shapes; Structure; Surface; Synapses; Techniques; Time; Transgenic Mice; Viral; Viral Vector; dextran; improved; in vivo; novel; olfactory bulb; olfactory receptor; olfactory stimulus; optical imaging; public health relevance; receptor; recombinase; research study; response; selective expression; sensor; spatiotemporal; voltage

DESCRIPTION (provided by applicant): The ability to detect and discriminate between thousands of different odorants begins with olfactory stimuli activating ensembles of receptor neurons that send axonal projections terminating in neuropil regions called glomeruli located on the surface of the olfactory bulb. Mitral/tufted neurons are the primary output neurons of the olfactory bulb, and have primary apical dendrites that extend into the glomerular tufts that are innervated by the olfactory receptor axon terminals. Different odorants activate spatially distinct patterns of glomeruli across the surface of the olfactory bulb. This input organization has been characterized for a wide range of odorants and concentrations. However, mitral/tufted neuron responses are shaped by two layers of inhibitory and excitatory interneurons, a complex arrangement that allows for many possible functions of the bulb. Here, we propose to simultaneously use calcium dyes loaded into sensory axons to examine the input to the bulb, and target the novel fast fluorescent protein voltage sensor ArcLight mitral/tufted neurons to examine the output. These experiments would be the first to simultaneously examine the spatio-temporal patterns of both the input and output of the olfactory bulb.

描述（由申请人提供）：检测和区分数千种不同气味的能力始于嗅觉刺激激活受体神经元的集合，所述受体神经元发送轴突投射，所述轴突投射终止于位于嗅球表面上的称为肾小球的神经元区域。二尖瓣/簇状神经元是嗅球的初级输出神经元，并且具有初级顶树突，其延伸到由嗅觉受体轴突终末支配的肾小球簇中。不同的气味激活空间上不同的 嗅球表面的小球图案。该输入组织的特征在于具有广泛的气味剂和浓度。然而，二尖瓣/簇状神经元的反应是由两层抑制性和兴奋性中间神经元形成的，这是一种复杂的排列，允许灯泡的许多可能的功能。在这里，我们建议同时使用加载到感觉轴突的钙染料来检查灯泡的输入，并针对新型快速荧光蛋白电压传感器ArcLight二尖瓣/簇状神经元来检查输出。这些实验将是第一个同时研究嗅球输入和输出的时空模式的实验。