Development of Point-of-Care Diagnostics for Norovirus

诺如病毒即时诊断的开发

• 批准号: 8839869
• 负责人: Suri Saranathan Iyer
• 金额: $ 44.75万
• 依托单位: GEORGIA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8839869
• 关键词: Accounting; Antibodies; Antigens; Antiviral Agents; Archives; Binding; Biological Assay; Biosensor; Blood Group Antigens; Capsid; Clinical; Detection; Development; Diagnostic; Discipline; Disease Outbreaks; Epithelial Cells; Equipment; Exhibits; Feces; Food; Gastroenteritis; Gastrointestinal tract structure; Generations; Genotype; Goals; Gold; Health care facility; Human; Human Resources; Infection; Lateral; Lead; Libraries; Link; Mucins; Nature; Norovirus; Patients; Performance; Phase; Polysaccharides; Positioning Attribute; Prevention; RNA Viruses; Research; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Scientist; Seasons; Sensitivity and Specificity; Side; Solutions; Structure; Study Section; Sulfhydryl Compounds; Surface; Testing; Therapeutic; Training; Ursidae Family; Vaccines; Viral; Virus; Water; analog; base; functional group; gastrointestinal; innovation; nanoparticle; novel; novel diagnostics; novel strategies; pathogen; point of care; point-of-care diagnostics; prospective; rapid diagnosis; user-friendly

Project Summary. Noroviruses, a group of nonenveloped single-stranded RNA viruses, are the leading cause of gastroenteritis outbreaks and account for 21 million cases in the US annually. Current prevention and control options are limited with no vaccines or therapeutics available. Antibody- based norovirus diagnostic kits suffer from low sensitivity and are dependent on the antibody used. Rapid, accurate, point of care diagnostic kits are critical to detect and isolate infected personnel. We propose a novel approach by using synthetic glycans as capture molecules. In this proposal, an interdisciplinary team will develop rapid, point of care diagnostics for the sensitive detection of norovirus. In Specific Aim 1, we will use a modular synthetic strategy to generate synthetic analogs of histo-blood group antigens and Lewis antigens, attachment factors for norovirus. In Specific Aim 2, we will develop and evaluate glycan based diagnostic platforms using panels of norovirus strains In Specific Aim 3, candidate diagnostic platforms will be compared side-by-side evaluated using clinical samples from both outbreaks and sporadic cases of gastrointestinal illness. Accomplishing the specific aims in this proposal is expected to yield rapid, robust, point of care, user friendly diagnostics for the precise and sensitive detection of all human norovirus strains.

项目摘要。 诺如病毒是一组无包膜的单链RNA病毒， 胃肠炎爆发的原因之一，每年在美国有2100万例病例。电流 由于没有疫苗或治疗方法，预防和控制的选择有限。抗体- 基于诺如病毒的诊断试剂盒灵敏度低， 采用快速、准确的即时诊断试剂盒对于检测和隔离感染者至关重要 人员的我们提出了一种新的方法，通过使用合成的聚糖作为捕获分子。在 根据这项提议，一个跨学科小组将为患者开发快速的护理点诊断， 诺如病毒的灵敏检测。在具体目标1中，我们将使用模块化合成策略， 产生组织血型抗原和刘易斯抗原的合成类似物， 诺如病毒的因素。在具体目标2中，我们将开发和评估基于聚糖的诊断 在特定目标3中，候选诊断平台将 使用来自暴发和散发的临床样本进行并排评估， 胃肠道疾病的病例。实现本提案中的具体目标预计将 产生快速、稳健、护理点、用户友好的诊断，用于精确和灵敏的检测 所有人类诺如病毒株。