Identification and Validation of Novel Human T cell Epitopes in Lassa Fever

拉沙热中新型人类 T 细胞表位的鉴定和验证

• 批准号: 8937428
• 负责人: MICHAEL B.A. OLDSTONE
• 金额: $ 142.02万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-30 至 2015-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8937428
• 关键词: Adult; Africa; Alleles; Award; Binding; Biological Assay; Bioterrorism; CD4 Positive T Lymphocytes; CD8B1 gene; Child; Communities; Computer software; Contracts; Data; Databases; Diagnostic; Emerging Communicable Diseases; Epitopes; Glycoproteins; Goals; Human; Human Volunteers; Immune; In Vitro; Lassa Fever; Lassa virus; Maps; Nucleoproteins; Patients; Peptides; Research; Sierra Leone; T-Cell Proliferation; T-Lymphocyte; T-Lymphocyte Epitopes; Validation; aged; enzyme linked immunospot assay; immunogenicity; microorganism; neonate; novel; programs; protein expression; tool; validation studies

The contracts awarded under the Large Scale T Cell Epitope Discovery program establish highly interactive multi-disciplinary teams whose research efforts are focused on large-scale discovery of T cell epitopes associated with microorganisms responsible for emerging and re-emerging infectious diseases and potential agents of bioterrorism. Projects are milestone driven and must include: 1) epitope identification; 2) in vitro epitope validation studies with primary human T cells to demonstrate the immunogenicity/antigenicity or diagnostic potential of all newly defined T cell epitopes shown to bind to classical or non-classical MHC molecules; and 3) submission of all epitope information and computer software to the Immune Epitope Database to facilitate access and use of the data and tools by the broader research community.

根据大规模T细胞表位发现计划授予的合同建立了高度互动的多学科团队，其研究工作重点是大规模发现与新出现和重新出现的传染病和生物恐怖主义潜在病原体有关的微生物相关的T细胞表位。项目是里程碑驱动的，必须包括：1）表位鉴定; 2）用原代人T细胞进行体外表位验证研究，以证明所有新定义的T细胞表位的免疫原性/抗原性或诊断潜力，这些表位显示与经典或非经典MHC分子结合;和3）向免疫表位数据库提交所有表位信息和计算机软件，以便于更广泛的研究访问和使用数据和工具社区