Signaling, Motility and TEM Assays

信号传导、运动和TEM检测核心的目的是提供方法学、实验评估， 分析和解释体外试验，以评估肿瘤和间质细胞的个体行为， 细胞及其相互作用。Core执行检测2D运动细胞行为、3D 侵袭和跨内皮迁移。这些测定允许在培养过程中对细胞行为进行完整的评价。 趋化性，侵袭和迁移穿过血管内皮，并提供了一个彻底的分析， 在昂贵和耗时的动物研究之前，我们已经研究了给定细胞类型的侵入能力。而且这些 体外试验支持和补充了多光子成像、侵入性 和TEM感受态细胞收集和分析核心（核心A）。核心B还充当程序化的 用于使用独特的蛋白质生物传感器和用于检测入侵足形成的测定的资源，因此 确保这些技术在所有项目中的应用是统一的。试验支持 通过核心将允许对肿瘤和基质细胞使用的分子机制进行急性分析， 促进肿瘤细胞的侵袭、内渗和外渗。 相关性（参见说明）： 核心B提供的服务对于计划项目的总体目标至关重要， 肿瘤和支持侵袭和转移的基质细胞之间的详细相互作用。这些体外 分析提供了关于细胞行为和机制的信息，这些信息对于解释细胞内 活体和活体成像研究。内核还协调使用独特的生化试剂， 各实验室开发的生物传感器和方法，分发给所有项目。

The aim of the Signaling, Motility and TEM Assays Core is to provide methodology, experimental evaluation, analysis and interpretation of in vitro assays in order to assess the individual behavior of tumor and stromal cells as well as their interactions. The Core performs assays examining 2D motile cell behavior, 3D invasion, and transendothelial migration. These assays permit a complete evaluation of cell behavior during chemotaxis, invasion and migration across a vascular endothelium, and provide a thorough analysis of the invasive capabilities of a given cell type prior to costly and time-consuming animal studies. Moreover, these in vitro assays support and complement the in vivo studies provided by the Multiphoton Imaging, Invasive and TEM Competent Cell Collection and Analysis Core (Core A). Core B also functions as a programmatic resource for the use of unique protein biosensors and for assays examining invadopod formation, and thus ensures that the application of these technologies is uniform across all of the projects. The assays supported by the Core will permit an acute analysis of the molecular mechanisms used by tumor and stromal cells to promote tumor cell invasion, intravasation and extravasation. RELEVANCE (See instructions): Services provided by Core B are essential for the overall objectives of the Program Project to define the detailed interactions between tumor and stromal cells that support invasion and metastasis. These in vitro assays provide information about cellular behavior and mechanism that is essential for the interpretation of in vivo and intravital imaging studies. The Core also coordinates the use of unique biochemical reagents, biosensors and methodologies developed by individual laboratories for distribution to all of the projects.