A genetic and genomic resource for vision research

用于视觉研究的遗传和基因组资源

• 批准号: 8723224
• 负责人: Sacha B Nelson
• 金额: $ 39.63万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8723224
• 关键词: Age; Behavior; Breeding; Data; Databases; Electrophysiology (science); Enhancers; Exhibits; Feedback; Gene Expression; Gene Expression Profile; Gene Targeting; Genes; Genetic; Genomics; Green Fluorescent Proteins; Human; Inferior Colliculus; Knock-out; Label; Libraries; Link; Mammals; Maps; Measures; Methods; Molecular; Molecular Profiling; Morphology; Mouse Strains; Mus; Neurons; Output; Pathway interactions; Pattern; Phenotype; Physiological; Physiology; Plant Roots; Population; Property; Proteins; Reporter; Research Personnel; Resources; Rodent; Role; Somatosensory Cortex; Specific qualifier value; Structure; Synapses; Synaptic plasticity; System; Tetanus Helper Peptide; Transgenic Mice; Transgenic Organisms; Vision research; Visual; Visual Cortex; Visual system structure; area striata; cell type; critical period; deprivation; experience; genetic manipulation; genome-wide; hippocampal pyramidal neuron; innovation; interest; knockout gene; monocular deprivation; novel; overexpression; promoter; recombinase; response; scale up; superior colliculus Corpora quadrigemina; tool; transgene expression

DESCRIPTION (provided by applicant): Genetic manipulation of the mammalian visual system is currently limited by available tools for targeting gene knockout or overexpression to specific neuronal cell types, and is further hampered by an incomplete understanding of the normal gene expression in those cell types. This proposed studies will use a novel genetic strategy, lentiviral enhancer trapping, to generate new driver strains of mice expressing cre recombinase and tet activator in specific cell types. Recently developed cell type specific expression profiling will be used to map gene expression in these and other neurons within the central visual system. The genetic and genomic tools and resources developed will then be used to study the cellular and molecular mechanisms underlying experience dependent plasticity in layer 4 input neurons and layer 6 output neurons of the primary visual cortex.

描述（由申请人提供）：哺乳动物视觉系统的遗传操作目前受到用于将基因敲除或过表达靶向特定神经元细胞类型的可用工具的限制，并且进一步受到对这些细胞类型中正常基因表达的不完全理解的阻碍。这项研究将使用一种新的遗传策略，慢病毒增强子捕获，以产生在特定细胞类型中表达cre重组酶和泰特激活剂的新的小鼠驱动株。最近开发的细胞类型特异性表达谱将用于绘制这些和其他神经元中的基因表达在中央视觉系统。遗传和基因组的工具和资源开发，然后将被用来研究的细胞和分子机制的基础上的经验依赖可塑性的第4层输入神经元和第6层输出神经元的初级视觉皮层。