Point of care microfluidic diagnosis system for rapid and sensitive detection of
用于快速、灵敏检测的护理点微流体诊断系统
基本信息
- 批准号:8507118
- 负责人:
- 金额:$ 73.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-07-01 至 2015-06-30
- 项目状态:已结题
- 来源:
- 关键词:AdenovirusesAmericanAnti-Bacterial AgentsAntibiotic ResistanceAntibioticsAntiviral AgentsAntiviral resistanceAvian InfluenzaAwardBedside TestingsBioinformaticsBiological AssayBirdsCaliforniaCanadaCategoriesChemistryClinicalClinical VirologyCommunicable DiseasesCommunicationDNADNA amplificationDataDatabasesDetectionDevelopmentDevicesDiagnosisDiagnosticDiagnostic ProcedureDiagnostic testsEnsureFluorescenceGenesGenomicsGoalsHealthHealth Information SystemHealth TechnologyHealthcare SystemsHospitalsHourHumanIndustryInfluenzaInfluenza A Virus, H1N1 SubtypeInfluenza A Virus, H5N1 SubtypeInstitutesLabelLasersMeasuresMechanicsMedicalMethodsMicrofabricationMicrofluidic MicrochipsMicrofluidicsMolecularNational Institute of Allergy and Infectious DiseaseNational Research CouncilNucleic AcidsOne-Step dentin bonding systemOpticsParainfluenzaPatientsPharmacologic SubstancePlasticsPolymerase Chain ReactionPolymersPreclinical TestingPreparationPrizeProceduresProcessProduct ApprovalsPublic HealthQuarantineRNARNA amplificationReactionReadingReagentResearchResearch PersonnelRespiratory SystemRespiratory Tract InfectionsRespiratory tract structureReverse Transcriptase Polymerase Chain ReactionSARS coronavirusSamplingScienceScientistSocietiesSpecialistSpecimenSurfaceSwabSystemTechnologyTechnology TransferTest ResultTimeUnited States National Institutes of HealthUniversitiesValidationViralViral AntigensViral Respiratory Tract InfectionVirusWorkauthoritybasebiodefensecontagioncostcost effectivedensitydesignfluimprovedinstrumentinstrumentationinternal controlmeetingsmicro-total analysis systemmicrobialnanofabricationnovel diagnosticspandemic diseasepathogenpoint of carepoint-of-care diagnosticspre-clinicalpreventproduct developmentresearch clinical testingrespiratory virusresponsesample collectionswine flutoolviral RNAvirology
项目摘要
DESCRIPTION (provided by applicant): The objective of this proposal is to merge genomics, microfluidics, DNA and RNA amplification, microarrays, analytical instrumentation development and polymer microfabrication expertises to develop a revolutionary medical diagnostic device for the rapid multiparametric detection of the most relevant viruses found in respiratory tract clinical samples. Viral respiratory tract infection (VRTI) is the most common illness today (Fendrick et al., 2003), and costs over $50 billion yearly in the US alone. It is estimated that more than a dozen different viruses infect human upper airways, yet current diagnostic methods based on viral antigens often lack the required sensitivity, while culture-based methods are extremely slow. Universit¿ Laval with its collaborators intends to integrate their already existing diagnostic technologies into a single Microfluidic Centripetal Device (MCD), or Lab-on-a-Chip, for the detection of VRTIs (VRTI-MCD). The VRTI-MCD will automatically (i) process nasopharyngeal swab samples, (ii) extract viral RNA/DNA in a sufficiently pure form, (iii) amplify and label nucleic acids, (iv) process labelled-amplicons, (v) hybridize amplicons on low density microarrays, (vi) read and interpret the data to provide a clinically useful result within one hour, and (vii) communicate results in real-time to health information systems. The VRTI-MCD will be a disposable polymer-based device that will encase all necessary reagents. The disposable will be processed by a portable and easy-to-use opto-mechanical instrument enabling automated VRTI diagnostics. Seventeen viruses, including influenza A (such as avian flu and swine flu), influenza B, SARS coronavirus, parainfluenza, and adenovirus responsible for the majority of VRTI along with key antiviral resistance markers will be detectable using a single VRTI-MCD. This novel diagnostic platform will be developed and optimized using nasopharyngeal swab specimens available in the extensive clinical samples collection at the Centre de Recherch¿ en Infectiologie (CRI). By the end of year-five support by NIH, we will have validated the integrated VRTI-MCD using human clinical samples and the portable instrument will be ready for worldwide clinical testing for diagnostics at the point-of-care. Thereafter, within two years, product development and approval by health authorities (FDA, Health Canada, etc.) will be undertaken by a commercial partner. This new technological platform will result in an unprecedented expansion of point-of-care diagnostic tests (POCTs) for viral respiratory tract infections, while providing rapid yet economical molecular diagnostics results in less than one hour instead of the current 2-3 days. This will improve medical response, allowing for the timely use of proper antiviral drugs and to ensure that rapid quarantine measures only be used when appropriate. These POCTs will help public health authorities to define new operational strategies for both lab-based and field-based applications.
描述(由申请人提供):本提案的目标是融合基因组学、微流体学、DNA 和 RNA 扩增、微阵列、分析仪器开发和聚合物微制造专业知识,开发一种革命性的医疗诊断设备,用于快速多参数检测呼吸道临床样本中发现的最相关病毒。病毒性呼吸道感染 (VRTI) 是当今最常见的疾病(Fendrick 等,2003),仅在美国每年造成的损失就超过 500 亿美元。据估计,有十多种不同的病毒会感染人类上呼吸道,但目前基于病毒抗原的诊断方法往往缺乏所需的灵敏度,而基于培养的方法则极其缓慢。拉瓦尔大学及其合作者打算将其现有的诊断技术集成到单个微流体向心装置 (MCD) 或芯片实验室中,用于检测 VRTI (VRTI-MCD)。 VRTI-MCD 将自动 (i) 处理鼻咽拭子样本,(ii) 提取足够纯的病毒 RNA/DNA,(iii) 扩增并标记核酸,(iv) 处理标记的扩增子,(v) 在低密度微阵列上杂交扩增子,(vi) 读取并解释数据,在一小时内提供临床有用的结果,以及 (vii) 将结果实时传达给卫生信息系统。 VRTI-MCD 将是一种一次性聚合物设备,可容纳所有必要的试剂。一次性产品将由便携式且易于使用的光机械仪器进行处理,从而实现自动 VRTI 诊断。使用单个 VRTI-MCD 可以检测 17 种病毒,包括甲型流感(如禽流感和猪流感)、乙型流感、SARS 冠状病毒、副流感和腺病毒,这些病毒是造成大多数 VRTI 的原因以及关键的抗病毒耐药标记。该新型诊断平台将使用感染研究中心 (CRI) 收集的大量临床样本中提供的鼻咽拭子样本进行开发和优化。到 NIH 支持的第五年结束时,我们将使用人体临床样本验证集成 VRTI-MCD,并且便携式仪器将准备好进行全球床旁诊断临床测试。此后,在两年内,产品开发和卫生当局(FDA、加拿大卫生部等)的批准将由商业合作伙伴承担。这一新技术平台将导致病毒性呼吸道感染的即时诊断测试 (POCT) 得到前所未有的扩展,同时在不到一小时(而不是目前的 2-3 天)内提供快速且经济的分子诊断结果。这将改善医疗反应,以便及时使用适当的抗病毒药物,并确保仅在适当时使用快速检疫措施。这些 POCT 将帮助公共卫生当局为实验室和现场应用制定新的操作策略。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michel G. Bergeron其他文献
Preventing Antibiotic Resistance Using Rapid DNA-Based Diagnostic Tests
使用基于 DNA 的快速诊断测试预防抗生素耐药性
- DOI:
- 发表时间:
1998 - 期刊:
- 影响因子:0
- 作者:
Michel G. Bergeron;Marc Ouellette - 通讯作者:
Marc Ouellette
Benefit from high intrarenal levels of gentamicin in the treatment of <em>E. coli</em> pyelonephritis
- DOI:
10.1038/ki.1986.211 - 发表时间:
1986-10-01 - 期刊:
- 影响因子:
- 作者:
Michel G. Bergeron;Yves Marois - 通讯作者:
Yves Marois
Comparison of rectal swabs and fecal samples for the detection of Clostridioides difficile infections with a new in-house PCR assay.
使用新的内部 PCR 检测方法比较直肠拭子和粪便样本,以检测艰难梭菌感染。
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:3.7
- 作者:
A. Huletsky;Vivian G Loo;Y. Longtin;Jean Longtin;Sylvie Trottier;Cécile L Tremblay;R. Gilca;Christian Lavallée;Éliel Brochu;Ève Bérubé;M. Bastien;M. Bernier;M. Gagnon;Johanne Frenette;J. Bestman;Louise Deschênes;Michel G. Bergeron - 通讯作者:
Michel G. Bergeron
A Pre–Phase III Efficacy Trial of the Spermicide/Contraceptive Effect of the Invisible Condom, a Non-Hormonal Vaginal Gel, in Women from Canada
- DOI:
10.1016/j.jogc.2021.09.018 - 发表时间:
2022-02-01 - 期刊:
- 影响因子:
- 作者:
Rabeea F. Omar;Mathieu Leboeuf;Madeleine Lemyre;Isabelle Chabot;Sylvie Trottier;André Ulmann;Michel G. Bergeron - 通讯作者:
Michel G. Bergeron
Comparison of rectal swabs and fecal samples for the detection of emClostridioides difficile/em infections with a new in-house PCR assay
使用新的内部聚合酶链反应检测法对直肠拭子和粪便样本检测艰难梭菌感染的比较
- DOI:
10.1128/spectrum.00225-24 - 发表时间:
2024-04-22 - 期刊:
- 影响因子:3.800
- 作者:
Ann Huletsky;Vivian G. Loo;Yves Longtin;Jean Longtin;Sylvie Trottier;Cécile L. Tremblay;Rodica Gilca;Christian Lavallée;Éliel Brochu;Ève Bérubé;Martine Bastien;Marthe Bernier;Martin Gagnon;Johanne Frenette;Julie Bestman-Smith;Louise Deschênes;Michel G. Bergeron;Anne L. Wyllie - 通讯作者:
Anne L. Wyllie
Michel G. Bergeron的其他文献
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{{ truncateString('Michel G. Bergeron', 18)}}的其他基金
Point of care microfluidic diagnosis system for rapid and sensitive detection of
用于快速、灵敏检测的护理点微流体诊断系统
- 批准号:
7939372 - 财政年份:2010
- 资助金额:
$ 73.75万 - 项目类别:
Point of care microfluidic diagnosis system for rapid and sensitive detection of
用于快速、灵敏检测的护理点微流体诊断系统
- 批准号:
8695281 - 财政年份:2010
- 资助金额:
$ 73.75万 - 项目类别:
Point of care microfluidic diagnosis system for rapid and sensitive detection of
用于快速、灵敏检测的护理点微流体诊断系统
- 批准号:
8301481 - 财政年份:2010
- 资助金额:
$ 73.75万 - 项目类别:
Point of care microfluidic diagnosis system for rapid and sensitive detection of
用于快速、灵敏检测的护理点微流体诊断系统
- 批准号:
8102954 - 财政年份:2010
- 资助金额:
$ 73.75万 - 项目类别:
Microfluidic system for point of care diagnosis of GBS
用于 GBS 护理点诊断的微流控系统
- 批准号:
6943106 - 财政年份:2004
- 资助金额:
$ 73.75万 - 项目类别:
Microfluidic system for point of care diagnosis of GBS
用于 GBS 护理点诊断的微流控系统
- 批准号:
7492217 - 财政年份:2004
- 资助金额:
$ 73.75万 - 项目类别:
Microfluidic system for point of care diagnosis of GBS
用于 GBS 护理点诊断的微流控系统
- 批准号:
7120084 - 财政年份:2004
- 资助金额:
$ 73.75万 - 项目类别:
Microfluidic system for point of care diagnosis of GBS
用于 GBS 护理点诊断的微流控系统
- 批准号:
6804254 - 财政年份:2004
- 资助金额:
$ 73.75万 - 项目类别:
Microfluidic system for point of care diagnosis of GBS
用于 GBS 护理点诊断的微流控系统
- 批准号:
7285980 - 财政年份:2004
- 资助金额:
$ 73.75万 - 项目类别:
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