Light encoding properties of wiry-type and starburst amacrine cells of the primat

项目摘要 所提出的研究的总体目标是确定光编码特性 灵长类视网膜中两类无长突细胞的形态结构：星形细胞和星形细胞。 尽管在研究细胞类型和神经回路方面取得了巨大进展， 在哺乳动物视网膜中，基本上对无长突细胞的功能一无所知。 灵长类视网膜第一次录音将进行从mandy型和星爆 猕猴视网膜体外制备物中的无长突细胞。 拟议的研究有两个具体目标： 1)目的：研究人视网膜神经节细胞中无长突细胞的光编码和内在特性。 灵长类动物的视网膜我将在2010年首次记录已鉴定的无长突细胞， 灵长类动物视网膜，并使用运动刺激和时空白色噪声来测量 这些细胞的编码特性。我将测试具体的假设（A），金属丝- 型细胞偏好局部运动而非整体运动，（B）电压门控钠通道 有助于信号在其树突中的传播，以及（C）树突型细胞是 GABA能 2)探讨灵长类动物星突无长突细胞是否具有方向选择性。我 将首次记录灵长类动物视网膜中已鉴定的星状无长突细胞 并利用运动刺激和空间噪声来直接确定灵长类动物的星爆 细胞具有方向选择性。我将具体检验假设（A），即灵长类动物 星爆细胞表现出方向选择性，更喜欢离心运动和（B）， 灵长类动物和小鼠的星状细胞显示出相似的光编码特性。

Project Summary The overall goal of the proposed research is to determine the light encoding properties of two classes of amacrine cell in the primate retina: wiry-type and starburst cells. Despite the tremendous advances in the study of cell types and circuits in the mammalian retina, essentially nothing is known of the function of amacrine cells in the primate retina. The first recordings will be performed from wiry-type and starburst amacrine cells in an in vitro preparation of the macaque monkey retina. The proposed research has two specific aims: 1) To determine the light encoding and intrinsic properties of wiry-type amacrine cells in the primate retina. I will make the first recordings of identified wiry-type amacrine cells in the primate retina and use motion stimuli and spatio-temporal white noise to measure the encoding properties of these cells. I will test the specific hypotheses (A) that wiry- type cells prefer local motion to global motion, (B) that voltage-gated sodium channels contribute to signal propagation in their dendrites, and (C) that wiry-type cells are GABAergic. 2) To determine whether primate starburst amacrine cells show direction selectivity. I will make the first recordings of identified starburst amacrine cells in the primate retina and use motion stimuli and spatial noise to determine directly whether primate starburst cells are direction selective. I will specifically test the hypotheses (A) that primate starburst cells show direction selectivity, preferring centrifugal motion and (B) that starburst cells in the primate and mouse show similar light encoding properties.