Characterization of an extracelluar structure produced by Staphylococcus aureus

金黄色葡萄球菌产生的细胞外结构的表征

• 批准号: 8584254
• 负责人: BLAISE R BOLES
• 金额: $ 19.01万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8584254
• 关键词: Amino Acid Sequence; Amyloid fibers; Animal Model; Cell Wall; Cells; Communicable Diseases; Data; Development; Electrons; Environment; Exposure to; Extracellular Structure; Fiber; Growth; Health; Human; Individual; Infection; Integration Host Factors; Knowledge; Laboratories; Life; Microbial Biofilms; Molecular; Nose; Peptide Hydrolases; Peptides; Phenols; Population; Regulation; Resistance; Risk Factors; Rodent Model; Role; Staphylococcus aureus; Structure; Toxin; Translating; United States; Vision; base; beta pleated sheet; cost; extracellular; mutant; novel; pathogen; prevent; public health relevance; surfactant

DESCRIPTION (provided by applicant): Staphylococcus aureus is a medically important human pathogen that is found in the nasal passages of approximately 1/3 of the human population. The nose serves as a reservoir for spread of this pathogen and predisposes the host to potential infection. Despite the importance of S. aureus to human health, molecular mechanisms and host factors influencing nasal colonization are not well understood. We have recently discovered an extracellular amyloid fiber produced by S. aureus during nasal colonization. This finding is extremely novel and exciting as S. aureus has never been shown to produce extracellular structures. We hypothesize that this extracellular fiber is important for S. aureus nasal colonization and persistence within the nose. Our preliminary results suggest that the fibers contribute to biofilm development and consist of multiple toxins, which are folding into beta sheet rich confirmations to self-assemble into amyloid fibers. The aims of this proposal will determine the composition, formation, degradation and role of S. aureus fibers during nasal colonization and biofilm development. At the conclusion of these studies, we will have expanded our knowledge of S. aureus nasal colonization and characterized a novel extracellular structure produced by this important commensal and pathogen.

描述（由申请方提供）：金黄色葡萄球菌是一种医学上重要的人类病原体，存在于约1/3人群的鼻道中。鼻子是这种病原体传播的储存库，使宿主易于受到潜在的感染。尽管S.金黄色葡萄球菌对人类健康的影响，影响鼻腔定植的分子机制和宿主因素尚不清楚。我们最近发现了由S.金黄色葡萄球菌在鼻腔定植。这一发现是非常新颖和令人兴奋的S。金黄色葡萄球菌从未显示出产生细胞外结构。我们推测这种细胞外纤维对S.金黄色葡萄球菌鼻内定植和鼻内持久性。我们的初步结果表明，纤维有助于生物膜的发展，并由多种毒素组成，这些毒素折叠成 富含β片层的证实，以自组装成淀粉样纤维。这一建议的目的将决定S的组成、形成、降解和作用。金黄色葡萄球菌纤维在鼻腔定植和生物膜的发展。在这些研究的结论，我们将扩大我们的知识，S。金黄色葡萄球菌鼻腔定植和特点的新的细胞外结构产生的这一重要的真菌和病原体。