CORE E: Tissue Culture

动物和人类细胞系，特别是来自患者的细胞系，对于分离大量的 基因组DNA和RNA，并用于制备蛋白质提取物用于蛋白质印迹研究以及酶 分析.基因组DNA可以从分离自全血的淋巴细胞制备。但 来自患者的永生化B淋巴母细胞或原代皮肤成纤维细胞的可用性特别 有利的几个原因。细胞系提供了一种可靠的手段，确保额外的DNA， 这是必要的，而不会在额外的静脉穿刺中打扰患者和家庭成员。这也是一种保障， 特别是在病态（致命）疾病的情况下，以确保致病的DNA、RNA和蛋白质来源 研究和重复分析时，新的信息和技术变得可用。此外，Cell 在涉及瞬时和长期表达的研究中，线用于电穿孔和病毒感染。 最后，来自IDD患者的成纤维细胞可能在将来被证明对诱导成多能细胞有价值。 机制研究和潜在的治疗筛选。

Animal and human cell lines, particulariy those derived from patients, are crucial for isolating large quantities of genomic DNA and RNA, and for preparing protein extracts for Western blotting studies as well as enzyme analysis. Genomic DNA can be prepared from lymphocytes isolated from whole blood. However, the availability of immortalized B-lymphoblasts or primary skin fibroblasts from the patient is particularly advantageous for several reasons. Cell lines provide a reliable means of securing additional DNA when necessary without disturbing the patient and family members in additional venipunctures. It is also a safeguard, especially in the case of morbid (fatal) disease, to ensure DNA, RNA and protein sources for pathogenesis studies and for repeated analysis when new information and technology become available. In addition, cell lines are used for electroporation and viral infections in studies involving transient and long term expression. Lastly, fibroblasts from patients with IDD are likely to prove valuable in the future for induction into pluripotent mechanistic studies and potentially for screening for therapeutics.