Unnatural Amino Acid Mutagenesis with Fluorescent Amino Acids

荧光氨基酸的非天然氨基酸诱变

• 批准号: 8775602
• 负责人: Andrew Vito Anzalone
• 金额: $ 4.14万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8775602
• 关键词: Achievement; Amber; Amino Acids; Amino Acyl Transfer RNA; Attention; Behavior; Binding; Biochemical; Biochemistry; Biological; Biological Process; Biomedical Research; Cell Line; Cells; Cellular biology; Codon Nucleotides; Coupling; Data; Development; Discrimination; Enabling Factors; Eukaryotic Cell; Evaluation; Evolution; Extinction (Psychology); Generations; Genetic Code; Goals; Image; Imaging Techniques; In Vitro; Individual; Label; Laboratories; Lead; Libraries; Life; Ligase; Malignant Neoplasms; Mammalian Cell; Measurement; Molecular; Mutagenesis; Organism; Pathway interactions; Proteins; Reagent; Research; Research Personnel; Ribosomes; Saccharomyces cerevisiae; Site; Structural Models; Structure; System; Technology; Terminator Codon; Transfer RNA; Translations; Ursidae Family; analog; base; biophysical techniques; cellular imaging; design; directed evolution; fluorophore; human disease; improved; in vivo; insight; mutant; polypeptide; public health relevance; reconstitution; response; theories; tool

DESCRIPTION (provided by applicant): Cell-imaging and biophysical techniques have become integral components of basic biomedical research. These experimental approaches enable researchers to dissect dynamic biological pathways relevant to cell biology as a whole, and they have proven especially useful in elucidating the molecular mechanisms of diseased cells, such as those found in cancer. To help better examine these biological processes, we aim to develop a small, genetically encoded, high quality fluorescent tag for use in live-cell imaging and biophysical applications. We will combine structure-based design with precise biochemical analysis to identify probes that are compatible with the cellular machinery. Further steps will be taken to evaluate and implement the probes in living eukaryotic and mammalian cells. If successful, this technology would provide an alternative to the fluorescent proteins, which currently dominate the field, for a range of imaging and biophysical applications.

描述（由申请人提供）：细胞成像和生物物理技术已成为基础生物医学研究的组成部分。这些实验方法使研究人员能够剖析与整个细胞生物学相关的动态生物学途径，并且已被证明它们在阐明病变细胞（例如癌症中发现的细胞）的分子机制方面特别有用。为了帮助更好地研究这些生物过程，我们的目标是开发一种小的，遗传编码的，高质量的荧光标记，用于活细胞成像和生物物理应用。我们将结合联合收割机的结构为基础的设计与精确的生化分析，以确定探针是兼容的细胞机器。将采取进一步的步骤来评估和实施在活的真核和哺乳动物细胞中的探针。如果成功的话，这项技术将为目前在该领域占主导地位的荧光蛋白提供一种替代品，用于一系列成像和生物物理应用。