Intravital Microscopy Core

活体显微镜核心

• 批准号: 8590478
• 负责人: XANDRA OWENS BREAKEFIELD
• 金额: $ 41.45万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590478
• 关键词: Biogenesis; Biological; Biology; Brain; Brain Neoplasms; Cells; Communication; Data; Development; Devices; Diagnostic; Disease; Environment; Equipment; Evaluation; Extracellular Space; Functional RNA; Genes; Glioblastoma; Glioma; Health; Human Resources; Image; Imagery; Imaging Device; Imaging technology; Matrix Metalloproteinases; Mediating; Messenger RNA; Microscopy; Molecular; Monitor; Non-Malignant; Process; Production; Property; Reagent; Reporter; Research; Research Personnel; Role; Services; System; Techniques; Tumor-Derived; Vesicle; base; brain cell; brain tissue; computing resources; design; digital imaging; experience; extracellular; implantation; in vivo; innovation; insight; instrumentation; intercellular communication; intravital microscopy; mouse model; multi-photon; neoplastic cell; novel; programs; research study; tumor; uptake

Core Leader: Thorsten R. Mempel. The Intravital Microscopy Core (Core A) has been formulated based on a stated need of U19 investigators and is designed to draw on the considerable experience of its key personnel in multiphoton intravital microscopy (MP-IVM) techniques to provide expertise and specialized equipment and aid in the design and execution of in vivo imaging studies of exRNA- and EV-mediated communication of glioblastomata with their stromal environment and the surrounding, healthy brain tissue. The Specific Aims ofthe Intravital Microscopy Core are as follows: 1. To provide expertise in the design of specific experiments utilizing multi-photon microscopy-based analysis of EV biogenesis by glioblastomata, their properties in the extracellular space and uptake by nonmalignant brain cells, as well as of functional exRNA- and EV-mediated intercellular communication between tumor cells and tumor stroma. 2. To provide access to the required instrumentation and to aid in the execution of MP-IVM experiments using mouse models of glioblastoma developed by the project Pis with assistance of the core as outlined in aim 1. 3. To provide computational resources forthe storage, processing, in-depth analysis, and interpretation of digital imaging data generated in aim 2.

核心负责人：Thorsten R. Mempel。基于U19研究者的指定需求，已制定了插入式显微镜核心（核心A） 旨在利用其在多光插入的主要人员的大量经验 显微镜（MP-IVM）技术，可提供专业知识和专业设备，并帮助设计和 执行ExRNA和EV介导的胶质性胶质瘤的交流的体内成像研究 基质环境和周围健康的脑组织。 插入显微镜核心的特定目的如下： 1。在使用基于多光子显微镜的特定实验设计方面提供专业知识 通过胶质母细胞教对EV生物发生的分析，它们在细胞外空间中的特性和非态度的吸收 脑细胞以及功能性exRNA和EV介导的细胞间通信 肿瘤细胞和肿瘤基质。 2。为提供所需仪器的访问并帮助执行MP-IVM实验 使用项目PI开发的胶质母细胞瘤的鼠标模型，并在核心的帮助下概述 目标1。 3。为存储，处理，深入分析和解释提供计算资源 AIM 2中生成的数字成像数据。