Intravital Microscopy Core

活体显微镜核心

• 批准号: 8590478
• 负责人: XANDRA OWENS BREAKEFIELD
• 金额: $ 41.45万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590478
• 关键词: Biogenesis; Biological; Biology; Brain; Brain Neoplasms; Cells; Communication; Data; Development; Devices; Diagnostic; Disease; Environment; Equipment; Evaluation; Extracellular Space; Functional RNA; Genes; Glioblastoma; Glioma; Health; Human Resources; Image; Imagery; Imaging Device; Imaging technology; Matrix Metalloproteinases; Mediating; Messenger RNA; Microscopy; Molecular; Monitor; Non-Malignant; Process; Production; Property; Reagent; Reporter; Research; Research Personnel; Role; Services; System; Techniques; Tumor-Derived; Vesicle; base; brain cell; brain tissue; computing resources; design; digital imaging; experience; extracellular; implantation; in vivo; innovation; insight; instrumentation; intercellular communication; intravital microscopy; mouse model; multi-photon; neoplastic cell; novel; programs; research study; tumor; uptake

Core Leader: Thorsten R. Mempel. The Intravital Microscopy Core (Core A) has been formulated based on a stated need of U19 investigators and is designed to draw on the considerable experience of its key personnel in multiphoton intravital microscopy (MP-IVM) techniques to provide expertise and specialized equipment and aid in the design and execution of in vivo imaging studies of exRNA- and EV-mediated communication of glioblastomata with their stromal environment and the surrounding, healthy brain tissue. The Specific Aims ofthe Intravital Microscopy Core are as follows: 1. To provide expertise in the design of specific experiments utilizing multi-photon microscopy-based analysis of EV biogenesis by glioblastomata, their properties in the extracellular space and uptake by nonmalignant brain cells, as well as of functional exRNA- and EV-mediated intercellular communication between tumor cells and tumor stroma. 2. To provide access to the required instrumentation and to aid in the execution of MP-IVM experiments using mouse models of glioblastoma developed by the project Pis with assistance of the core as outlined in aim 1. 3. To provide computational resources forthe storage, processing, in-depth analysis, and interpretation of digital imaging data generated in aim 2.

核心领导人：Thorsten R. Mempel。活体显微镜核心（核心 A）是根据 U19 研究人员的既定需求而制定的 旨在借鉴其关键人员在多光子活体治疗方面的丰富经验 显微镜 (MP-IVM) 技术提供专业知识和专用设备以及设计和帮助 执行胶质母细胞瘤与 exRNA 和 EV 介导的通讯的体内成像研究 基质环境和周围的健康脑组织。 活体显微镜核心的具体目标如下： 1. 提供利用基于多光子显微镜的特定实验设计的专业知识 分析胶质母细胞瘤的 EV 生物发生、其在细胞外空间的特性以及非恶性细胞的摄取 脑细胞，以及功能性 exRNA 和 EV 介导的细胞间通讯 肿瘤细胞和肿瘤基质。 2. 提供对所需仪器的访问并帮助执行 MP-IVM 实验 使用由 Pis 项目开发的胶质母细胞瘤小鼠模型，并在核心的帮助下，如 目标1. 3. 为数据的存储、处理、深入分析和解释提供计算资源 目标 2 中生成的数字成像数据。