Intravital Microscopy Core

活体显微镜核心

• 批准号: 8590478
• 负责人: XANDRA OWENS BREAKEFIELD
• 金额: $ 41.45万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590478
• 关键词: Biogenesis; Biological; Biology; Brain; Brain Neoplasms; Cells; Communication; Data; Development; Devices; Diagnostic; Disease; Environment; Equipment; Evaluation; Extracellular Space; Functional RNA; Genes; Glioblastoma; Glioma; Health; Human Resources; Image; Imagery; Imaging Device; Imaging technology; Matrix Metalloproteinases; Mediating; Messenger RNA; Microscopy; Molecular; Monitor; Non-Malignant; Process; Production; Property; Reagent; Reporter; Research; Research Personnel; Role; Services; System; Techniques; Tumor-Derived; Vesicle; base; brain cell; brain tissue; computing resources; design; digital imaging; experience; extracellular; implantation; in vivo; innovation; insight; instrumentation; intercellular communication; intravital microscopy; mouse model; multi-photon; neoplastic cell; novel; programs; research study; tumor; uptake

Core Leader: Thorsten R. Mempel. The Intravital Microscopy Core (Core A) has been formulated based on a stated need of U19 investigators and is designed to draw on the considerable experience of its key personnel in multiphoton intravital microscopy (MP-IVM) techniques to provide expertise and specialized equipment and aid in the design and execution of in vivo imaging studies of exRNA- and EV-mediated communication of glioblastomata with their stromal environment and the surrounding, healthy brain tissue. The Specific Aims ofthe Intravital Microscopy Core are as follows: 1. To provide expertise in the design of specific experiments utilizing multi-photon microscopy-based analysis of EV biogenesis by glioblastomata, their properties in the extracellular space and uptake by nonmalignant brain cells, as well as of functional exRNA- and EV-mediated intercellular communication between tumor cells and tumor stroma. 2. To provide access to the required instrumentation and to aid in the execution of MP-IVM experiments using mouse models of glioblastoma developed by the project Pis with assistance of the core as outlined in aim 1. 3. To provide computational resources forthe storage, processing, in-depth analysis, and interpretation of digital imaging data generated in aim 2.

核心领导：Thorsten R. Mempel。活体显微镜核心（核心A）是根据U19研究者的既定需求制定的