Transgenic Mice for Hybrid Voltage Sensor Imaging of Neural Circuitry

描述（由申请人提供）：混合电压传感器（hVOS）技术使研究人员能够使用绿色荧光蛋白和其他荧光蛋白对膜电位进行成像。由于hVOS探针是由DNA编码的，因此可以使用遗传技术将它们定位到特定类型的细胞。该项目将培养具有hVOS探针的小鼠，该探针针对小鼠大脑中特定类型的神经元。hVOS探针将在多种脑区和细胞类型中表达。动物也将被开发出来，可以用来瞄准非常特定的细胞群。从这些动物身上提取的大脑切片将在成像实验中非常有用。基本上，任何大脑区域和任何神经细胞类型都可以使用这项工作中提出的策略进行研究。有了这些动物，研究人员将能够同时研究大量细胞的电活动。本实验室开发的hVOS探针被发现优先靶向轴突。表达这种hVOS探针的动物将有助于轴突兴奋性的一般研究。

DESCRIPTION (provided by applicant): The hybrid voltage sensor (hVOS) technique enables researchers to use green fluorescent protein and other fluorescent proteins to image membrane potential. Because hVOS probes are encoded by DNA, genetic techniques can be used to target them to specific types of cells. This project will develop mice with hVOS probes targeted to specific types of neurons in the mouse brain. Animals will be developed with hVOS probes expressed in a wide variety of brain regions and cell types. Animals will also be developed that can be used to target very specific populations of cells. Slices of brain prepared from these animals will be very useful in imaging experiments. Essentially any brain region and any nerve cell type will be accessible to studies using the strategy advanced in this work. With these animals researchers will be able to study the electrical activity of large numbers of cells simultaneously. A hVOS probe developed in this laboratory was found to preferentially target axons. Animals expressing this hVOS probe will be useful in general studies of axonal excitability.