Cell Cycle Regulators in Diabetes and Obesity

糖尿病和肥胖症中的细胞周期调节剂

• 批准号: 8939629
• 负责人: Sushil Rane
• 金额: $ 36.07万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8939629
• 关键词: Address; Animal Model; Cell Cycle; Cell Cycle Progression; Cell Cycle Proteins; Cell Death; Data; Diabetes Mellitus; Diet; Energy Metabolism; Epidemiology; Exposure to; Fatty acid glycerol esters; Future Generations; Goals; Growth and Development function; Homeostasis; Investigation; Malignant Neoplasms; Metabolic; Mus; Mutation; Obesity; Obesity associated disease; Organ; Pathogenesis; Pathway interactions; Phenotype; Probiotics; Process; Proteins; Public Health; Role; Technology; Tissues; Transforming Growth Factor beta; Transgenic Mice; blood glucose regulation; cancer risk; cdc Genes; energy balance; glucose tolerance; mouse model

In this proposal we will use mouse models that have mutations in the Cell cycle gene loci. We are studying mechanisms of glucose tolerance and energy homeostasis by evaluating functions in different metabolic organs in mice harboring mutations in several cell cycle genes. The findings are revealing important role of Cdks and TGG-beta proteins in processes that modulate energy balance. We are employing tissue-specific conditional mice and transgenic mice to abalte or activate the expression of candidate cell cycle proteins using Cre-loxP technology. These mouse models are them charaterized to evalaute their glucose tolerance and energy expenditure phenotypes. Where appropriate we are challenging the mice with high-fat diet, excercise and/or cold exposure to further understand the role of cell cycle protein in energy metabolism.

在这个建议中，我们将使用在细胞周期基因位点中有突变的小鼠模型。我们正在通过评估几种细胞周期基因突变小鼠不同代谢器官的功能来研究糖耐量和能量稳态的机制。这些发现揭示了Cdks和tgg - β蛋白在调节能量平衡过程中的重要作用。我们正在使用组织特异性条件小鼠和转基因小鼠使用Cre-loxP技术来抑制或激活候选细胞周期蛋白的表达。这些小鼠模型是他们的特点，以评估他们的葡萄糖耐量和能量消耗表型。在适当的情况下，我们通过高脂肪饮食、运动和/或低温暴露对小鼠进行挑战，以进一步了解细胞周期蛋白在能量代谢中的作用。