UC Davis Shared Astrios Cell Sorter

加州大学戴维斯分校共享 Astrios 细胞分选机

• 批准号: 8826347
• 负责人: Barbara L. Shacklett
• 金额: $ 55.47万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-06 至 2016-04-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826347
• 关键词: Aerosols; Agriculture; California; Cell Separation; Cells; Color; Comprehensive Cancer Center; Containment; Cytometry; Ensure; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescent Probes; Gases; Housing; Institution; International; Laboratories; Lasers; Medical; Medical center; Plants; Policies; Primates; Proteins; Protoplasts; Recommendation; Research; Research Personnel; Research Support; Resource Sharing; Sampling; Services; Societies; Sorting - Cell Movement; Speed; System; United States National Institutes of Health; Universities; Veterinary Medicine; Water; aged; animal tissue; biological research; design; flexibility; human tissue; instrumentation; medical schools; meetings; microorganism; multidisciplinary; programs; success

 DESCRIPTION (provided by applicant): The University of California at Davis (UCD) is a diverse educational institution recognized for excellence in medical, veterinary and agricultural research. In 1998, the UCD Flow Cytometry Shared Resource Laboratory received S10 program support (S10-RR012964-01) to purchase a multi-laser, 10-color Cytomation MoFlo high- speed cell sorter. Specific features in the design of the MoFlo cell sorter are ideally suite for use in a shared resource lab, facilitating the analysis and sorting of a variety of cells from human and animal tissues, as well as microparticles, microorganisms, and plant cell protoplasts. For 15 years, the MoFlo has supported the research of UCD investigators across a wide range of multidisciplinary programs in Davis and at the UCD Medical Center in Sacramento, including the Schools of Medicine and Veterinary Medicine, NCI Comprehensive Cancer Center and the California National Primate Research Center. However, after years of reliable service, the original "legacy" MoFlo is now in need of replacement. Key component systems are aged and obsolete, have become unreliable and are expensive or impossible to maintain, particularly the water-cooled gas lasers. Further, the large size of the legacy MoFlo does not allow for enclosure in a biosafety containment cabinet and fails to meet the modern biosafety recommendations of the International Society for the Advancement of Cytometry and the newly approved NIH Biosafety Policy for Cell Sorters. The proposed replacement instrumentation, the Beckman Coulter AstriosEQ, offers modern components but uses the same stable, reliable fluidic design that is the hallmark of MoFlo cell sorters. The AstriosEQ will be equipped with 4 lasers and 16 photodetectors to detect commonly used fluorescent probes and proteins and will be housed in a biosafety containment hood with aerosol management to ensure safe sorting of biohazardous samples. The success of NIH supported biological research at UC Davis depends on the uninterrupted availability of a flexible, reliable, and safe cell sorter. The AstriosEQ willbe housed in, managed and maintained by the Davis campus Shared Flow Cytometry Laboratory so that UCD investigators have access to cell sorting instrumentation and flow cytometry expertise for years to come.

 描述（由申请人提供）：加州大学戴维斯分校（UCD）是一所多元化的教育机构，以其在医学，兽医和农业研究方面的卓越成就而闻名。在1998年，UCD流式细胞术共享资源实验室收到S10计划支持（S10-RR 012964 -01）购买多激光，10色Cytomation MoFlo高速细胞分选机。MoFlo细胞分选仪设计中的特定功能非常适合在共享资源实验室中使用，有助于分析和分选来自人类和动物组织的各种细胞，以及微粒，微生物和植物细胞原生质体。15年来，MoFlo一直支持戴维斯和萨克拉门托的UCD医学中心的各种多学科项目的UCD研究人员的研究，包括医学和兽医学院，NCI综合癌症中心和加州国家灵长类动物研究中心。然而，经过多年的可靠服务，原来的“遗产”MoFlo现在需要更换。关键部件系统已经老化和过时，变得不可靠，维修费用昂贵或无法维修，特别是水冷式气体激光器。此外，传统MoFlo的大尺寸不允许封闭在生物安全防护柜中，并且不符合国际细胞计数促进协会的现代生物安全建议和新批准的NIH细胞分选机生物安全政策。拟议的替代仪器Beckman Coulter AstriosEQ提供了现代化的组件，但使用了相同的稳定，可靠的流体设计，这是MoFlo细胞分选仪的标志。AstriosEQ将配备4个激光器和16个光电探测器，以检测常用的荧光探针和蛋白质，并将被安置在具有气溶胶管理的生物安全防护罩中，以确保生物危害样品的安全分选。NIH支持的加州大学戴维斯分校生物研究的成功取决于灵活、可靠和安全的细胞分选仪的不间断可用性。AstriosEQ将被安置在戴维斯校园共享流式细胞术实验室管理和维护，以便UCD研究人员可以在未来几年内获得细胞分选仪器和流式细胞术专业知识。