OUHSC Macromolecular X-ray Facility

OUHSC X射线核心设备(1998年启动，由NIH向Paul Weigel博士提供创意赠款)提供 为各个科布雷项目提供各种关键技术方面的专业知识和设备 蛋白质和核加合物的结晶学和小角X射线散射研究(SAXS)。这个 技术包括在室温下筛选晶体的衍射质量，晶体低温保护剂 筛选、衍射数据采集、衍射数据处理、空间群指定、结构 确定、结构细化、结构分析和图形准备。核心设施将是 升级到包括SAXS仪器和所需的X射线发生器升级，这将有第二个 可用于水晶工作的端口。新的发生器将有四倍大的光子流量和一个更小的 直径梁。这两个特征对于筛选常见的小的、弱衍射的晶体是至关重要的 科布雷研究项目。X光设备主任负责监督仪器的维护， 为JLS、Other Cobre安排用户日程，并培训用户了解SAXS和X射线晶体学 成员和OSBN成员。核心基金将由布莱恩·穆尔斯博士指导，并提供协助 来自联合导演Augen Pioszak博士和Franklin Hays博士。他们加起来有超过35年的 具有大分子结晶学经验。

The OUHSC X-ray Core Facility (initiated in 1998 with a NIH IDeA grant to Dr. Paul Weigel) provides expertise and equipment to the individual COBRE projects on a variety of techniques critical to crystallographic and small angle X-ray scattering studies (SAXS) of proteins and nucleic adds. The techniques include screening crystals for diffraction quality at room temperature, crystal cryo-protectant screening, diffraction data collection, diffraction data processing, space group assignment, structure determination, structure refinement, structure analysis, and figure preparation. The Core Facility will be upgraded to Include a SAXS instrument and the required X-ray generator upgrade which will have a second port available for crystal work. The new generator will have a fourfold greater flux of photons and a smaller diameter beam. Both features are critical for the screening the small, weakly diffracting crystals common in the COBRE research projects. The X-ray Facility Director oversees maintenance of the instruments, scheduling of users, and training users in both SAXS and X-ray crystallography for Jls, other COBRE members, and OSBN members. The Core Facility will be directed by Dr. Blaine Mooers with assistance from co-Directors Dr. Augen Pioszak and Dr. Franklin Hays. They have over 35 years of combined experience in macromolecular crystallography.