High-speed volumetric imaging of neuronal network activity at depth using Multiplexed Scanned Temporal Focusing (MuST)

使用多重扫描时间聚焦 (MuST) 对深度神经元网络活动进行高速体积成像

• 批准号: 9011561
• 负责人: Alipasha Vaziri
• 金额: $ 77.14万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9011561
• 关键词: Address; Animal Model; Area; Biological Neural Networks; Brain; Brain imaging; Calcium; Cells; Characteristics; Communities; Complex; Cortical Column; Data; Devices; Drosophila genus; Face; Functional Imaging; Goals; Health; Hippocampal Formation; Hippocampus (Brain); Human; Image; Image Analysis; Imaging technology; Instruction; Internet; Lasers; Light; Mechanics; Methods; Microscope; Microscopy; Monitor; Mus; Names; Neurons; Neurosciences; Optics; Performance; Photons; Physiologic pulse; Physiological; Population; Population Dynamics; Resolution; Rodent; Sampling; Scanning; Slice; Source Code; Speed; System; Techniques; Technology; Time; Tissue Sample; Tissues; Universities; Zebrafish; base; brain tissue; calcium indicator; density; design; imaging modality; imaging system; improved; in vivo; information processing; insight; instrumentation; novel; open source; operation; optical imaging; prototype; relating to nervous system; temporal measurement; tool; tool development; two-photon; usability; web site

 DESCRIPTION (provided by applicant): We propose to design and build a device that will push the boundaries of existing technology for imaging the activity of large-scale neuronal networks at high speed and single cell resolution. This will be done by using a Multiplexed Scanned Temporal Focusing (MuST) strategy in combination with laser systems with optimized pulse characteristics. Our approach will enable unbiased calcium imaging of unprecedentedly large V-FOVs (500x500x500um at 20Hz, or 1x1x0.7mm at 3Hz) at multi-hertz time resolution. Such an imaging system has the potential to revolutionize neuroscience, providing, for the first time, the ability to monitor the dynamics of network activity of tens of thousands of neurons near-simultaneously. In the mammalian cortex this capability provides the opportunity to gain insights into the computational principles for information processing as it will allow capturing an correlating the dynamics of the network activity across cortical layers. Our technology will be developed through an iterative and in a user-led fashion with our collaborator at Columbia University where it will be made accessible to the neuroscience community. The dissemination of this technique will be further facilitated by providing a dedicated website including open source code and detailed list of materials and instructions for its design and operation.

 描述（由申请人提供）：我们提出设计和构建一种设备，该设备将推动现有技术的边界，用于以高速和单细胞分辨率对大规模神经元网络的活动进行成像。这将通过使用多路复用扫描时间聚焦（MuST）策略与具有优化脉冲特性的激光系统相结合来实现。我们的方法将能够以多赫兹的时间分辨率对前所未有的大V-FOV（20 Hz时为500 x500 x500 um，或3 Hz时为1x1x0.7mm）进行无偏钙成像。这种成像系统有可能彻底改变神经科学，首次提供几乎同时监测数万个神经元网络活动动态的能力。在哺乳动物皮层中，这种能力提供了深入了解信息处理的计算原理的机会，因为它将允许捕获跨皮层的网络活动的动态。我们的技术将通过迭代和以用户为主导的方式与我们在哥伦比亚大学的合作者一起开发，在那里它将被神经科学界所使用。将通过提供一个专门网站，包括开放源代码和详细的材料清单以及设计和操作说明，进一步促进这一技术的传播。