LiquidCell EM System with Temperature Control for Biological Imaging
LiquidCell EM System with Temperature Control for Biological Imaging
用于生物成像的带温度控制的 LiquidCell EM 系统
批准号：
8779700
负责人：
MingQiang Yi
金额：
$14.82万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
项目状态：
已结题
起止时间：
2014-09-15 至 2016-06-14
关键词：
BiologicalBiological ProcessBiological SciencesBiological TestingBiomedical ResearchBiophysicsCell physiologyCellsCellular biologyChemistryDevelopmentElectron MicroscopyEnvironmentFutureImageIndividualInvestigationJournalsLeadLifeLiquid substanceMarketingMembraneMicrofluidic MicrochipsMicrofluidicsMicroscopyMolecularNeurosciencesPhasePhysiologicalPrincipal InvestigatorProcessProteinsReactionResolutionRewardsSamplingSchemeScienceSmall Business Innovation Research GrantStressStructureSurfaceSystemTechniquesTechnologyTemperatureTestingThickTransmission Electron MicroscopyWaterWorkaqueousbiological researchbiological systemscancer therapycell assemblychemical reactioncolloidal nanoparticlecommercializationdesignexperiencein vivoinnovationlight microscopymacromoleculemembernanometernovelpublic health relevanceresearch studysilicon nitridesuccesstoolultra high resolutionvirology
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DESCRIPTION (provided by applicant): One of the most challenging, yet highly rewarding tasks in biological research is to visualize with high spatial resolution the structure of molecula machines and their interactions in cellular function under physiological conditions, such as in 37 ¿C aqueous environment. This proposed LiquidCell EM system with precise temperature control enables direct imaging of many dynamic biological processes in truly native environments with ultra high resolution using transmission electron microscopy. In this proposal, we will fabricate the self-contained liquid cells with ultra-thin viewing windows. Precise temperature control of 37+/-0.1 ¿C will be achieved through microfluidic device fabrication. Test experiments of imaging individual proteins, protein assembly and live cell functions will be performed to optimize the system for in vivo biological imaging under physiological conditions.
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