An Orthogonal and Reversible Protein Labeling via Phosphopantetheinylation

 描述(由申请人提供)：蛋白质标记方法的进步是加速高含量显微技术、加快蛋白质组分析以及扩大我们识别和监测疾病的工具的关键。这个项目考察了一种可逆系统的发展，以标记选定的蛋白质。与传统方法不同，建议的系统采用肽筛选技术作为一种工具，在两步酶对反应中编码离散的选择性。选择性标记由标记酶的选择决定。提出的方案首先通过计算分析合理地开发正交标签。对该系统的评估包括详细的动力学分析和理解蛋白质复合体的应用。总体而言，该系统为蛋白质科学开辟了新的途径。该程序将提供一种新的方法，用于快速标记复杂蛋白质纲要中的特定蛋白质，以便将来在细胞或组织内进行研究。

 DESCRIPTION (provided by applicant): The advancement of protein labeling methodology is key to accelerating high-content microscopy technologies, expediting proteomic analyses, and expanding our tools to identify and monitor disease. This program examines the development of a reversible system to label selected proteins. Unlike conventional approaches, the proposed system adapts peptide-screening technologies as a tool to encode discrete selectivity within a two-stage enzymatic pair reaction. Selective labeling is dictated by the choice of labeling enzymes. The proposed program begins through the rational development of orthogonal tags by computational analysis. Evaluation of this system includes detailed kinetic analyses and applications for understanding protein complexes. Overall, this system opens new avenues in protein science. This program will provide a new method for rapidly labeling specific proteins within the complex compendium of proteins towards future studies within the cell or tissue.