Animal, Imaging, and Cytometry Core

动物、成像和细胞计数核心

基本信息

批准号：
8792578
负责人：
Kewal Asosingh
金额：
$ 18.24万
依托单位：
CLEVELAND CLINIC LERNER COM-CWRU
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8792578
关键词：
Abbreviations Allergens Allergic Animal Experiments Animals Asthma Biological Models Biology Breeding Cells Complex Cyclic AMP Cytometry Data Databases Electroporation Emission-Computed Tomography Figs - dietary Functional Imaging Gene Proteins Generations Genes Histologic IACUC Image Image Cytometry Knock-out Knockout Mice Lung Maintenance Measurement Mitochondria Modeling Mouse Strains Mus NOS1 gene NOS2A gene NOS3 gene Nitric Oxide Nuclear Ovalbumin Oxidoreductase Pathway interactions Performance Phosphatidylinositols Phosphorylation Phosphotransferases Photons Positron-Emission Tomography Procedures Process Process Measure Pulmonary Inflammation Pyroglyphidae Radiation Radio Radioactive Receptor Signaling Research Safety Sampling Services Smooth Muscle Soluble Guanylate Cyclase Structure of parenchyma of lung Tissues airway hyperresponsiveness airway inflammation animal imaging arginase beta-adrenergic receptor cost in vivo innovation instrument knockout animal molecular imaging programs pyroglyphid research study success tissue processing

项目摘要

ABSTRACT - CORE D The Animal Imaging and Cytometry Core will support all Projects of the Program by providing key services, including: generation, breeding, and maintenance of genetically modified mouse colonies, murine asthma models (ovalbumin allergen, dust mite, and intratracheal IL-25 and in vivo NOS electroportion), mouse airway hyper-reactivity measures, processing and histological analyses of mouse lung tissues, murine data repository, in vivo-molecular imaging by Positron Emission Tomography (PET) and Single Photon Emission Computerized Tomography (SPECT) for iNOS activity and adherent cell cytometric analyses for intracellular probes. All required instruments and expertise are found in the Core. The Core expedites results and reduces cost of Projects through the use of shared model systems. New model systems and functional imaging increase overall innovation of the Program.

摘要 - 核心D 动物成像和细胞仪核心将通过提供关键服务，以支持该计划的所有项目，包括：生成，繁殖和维持转基因小鼠菌落，鼠哮喘型号（椭圆蛋白过敏原，灰尘和气管内IL-25和体内电力型），小鼠气道小鼠肺组织的高反应性测量，加工和组织学分析，鼠数据存储库，正电子发射断层扫描（PET）和单个光子发射计算机的体内分子成像用于iNOS活性和细胞内探针的依从性细胞仪分析的层析成像（SPECT）。全部所需的工具和专业知识在核心中找到。核心加快结果并降低了成本通过使用共享模型系统的项目。新的模型系统和功能成像增加该计划的整体创新。