Shared Resource: Biobanking and Correlative Sciences

项目摘要/摘要 生物库和相关科学(BCS)核心的目的是加强同行评审， KCI成员资助的研究活动，其研究需要获得IRB批准的患者组织用于 临床前和临床研究并通过管理组织标本和提供 循环肿瘤细胞计数。BCS核心被归入交叉学科研究核心集群 除了BCS核心，还包括生物统计学、基因组学和药理学核心。 BCS Core为KCI调查人员提供高质量、注释良好的肿瘤组织标本，用于预 临床研究和协调对肿瘤组织的要求与基因组学，药理学和 蛋白质组学是核心。疾病特定部位的病理学家检查组织以选择合适的样本。 这确保了特定研究所需的数量和数量上有适当和充足的组织 以提供由生物统计核心确定的具有生物学意义的结果。机构资金支持 病理学家的贡献。 KCI生物库中的新鲜冷冻标本保存在8-80⁰C的冰柜中，这是温度 已监控并已备份应急电源。所有标本都有条形码，并储存在冰冻保险箱中 集装箱。安全柜存储福尔马林固定石蜡包埋(FFPE)标本和任何准备好的 从生物标本上取下来的玻片。每一种生物菌种都登录到KCI数据库OnCore®中，其中包含其精确的 位置、相关知情同意、病理报告和可用组织数量。组织学服务 由组织处理机、组织嵌入器、显微切割机、低温恒温器和其他辅助组织学支持 设备。对于药效学分析，核心包含Janssen细胞搜索循环肿瘤细胞 该系统使研究人员能够清点和/或分离循环中的肿瘤细胞。 生物库和相关科学核心提供的服务为21个同行- 审查了本审查期内的出版物。

PROJECT SUMMARY/ ABSTRACT The purpose of the Biobanking and Correlative Sciences (BCS) Core is to enhance the peer reviewed, funded research activities of KCI members whose research requires IRB-approved access to patient tissue for preclinical and clinical studies and to support clinical trials by managing tissue specimens and providing circulating tumor cell counting. The BCS Core is grouped in the Cross Disciplinary Research Core Cluster which, in addition to the BCS Core, includes the Biostatistics, Genomics, and Pharmacology Cores. The BCS Core provides KCI investigators with high quality, well-annotated tumor tissue specimens for pre- clinical studies and coordinates requests for tumor tissues with the Genomics, Pharmacology and Proteomics Cores. Disease site-specific pathologists review tissues for selection of appropriate samples. This ensures there is appropriate and adequate tissue in the amounts and numbers needed for a given study to provide biologically meaningful results as determined by the Biostatistics Core. Institutional funds support the contributions of the pathologists. Fresh frozen specimens in the KCI Biobank are stored in eight -80⁰C freezers, which are temperature monitored and have back up emergency power. All specimens are barcoded and stored in cryosafe containers. Secured cabinets store formalin-fixed paraffin embedded (FFPE) specimens and any prepared slides from the biospecimens. Each biospecimen is logged into the KCI database, OnCore®, with its precise location, associated informed consent, pathology report, and amount of available tissue. Histology services are supported by a tissue processor, a tissue embedder, microtomes, a cryostat, and other ancillary histology equipment. For pharmacodynamic assays, the core contains a Janssen Cell Search Circulating Tumor Cell system, which enables investigators to enumerate and/or isolate circulating tumor cells. The services provided by the Biobanking and Correlative Sciences Core have contributed to 21 peer- reviewed publications during the current review period.