Nutrient Interaction in Prostate Cancer Cells: Effects of I3C/DIM on AhR-mediated pathway

本协议的目的是在体外证实I3C/DIM对动物前列腺癌相关免疫细胞行为的影响，为细胞反应提供生理环境。研究设计包括使用多种分子生物学技术，包括DNA深度测序和转录组分析来表征机制。具体而言，本研究将在体内阐明I3C/DIM对巨噬细胞、Th17细胞等肿瘤相关免疫细胞影响的分子机制，并探讨I3C/DIM的防癌作用是否与免疫细胞介导的信号通路改变有关。

The goal of this agreement is to confirm in vitro findings of I3C/DIM effects on prostate cancer-associated immune cell behavior in animals that provide physiological environment for cellular response. The study design involves characterization of the mechanism using a variety of molecular biology techniques including DNA deep sequencing and transcriptome analyses. Specifically, this study will elucidate the molecular mechanism explaining the influence of I3C/DIM on tumor-associated immune cells including macrophages and Th17 cells in vivo and examine if the cancer preventive effects of I3C/DIM are associated with the changes in immune-cell mediated signaling pathways.