Dynamic Imaging of Retinal Microglia

视网膜小胶质细胞的动态成像

• 批准号: 9555687
• 负责人: Wai Wong
• 金额: $ 64.58万
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9555687
• 关键词: Age related macular degeneration; Aging; Anatomy; Behavior; Behavior Control; Blood Vessels; Cells; Choroid; Dyes; Exhibits; Fluorescence; Functional disorder; Goals; Image; Imaging Techniques; Immune; Immunologic Monitoring; Label; Microglia; Morphology; Mus; Myelogenous; Myeloid Cells; Nature; Pathogenesis; Physiology; Population; Positioning Attribute; Process; Reporting; Retina; Retinal; Retinal Diseases; Signal Transduction; Stimulus; Structure; Surface; Time; Tissues; Transgenic Mice; Transgenic Organisms; Trees; Work; age related; aged; arteriole; attenuation; cell motility; cell type; choroidal artery; density; extracellular; interest; macrophage; repaired; response; stellate cell; time use

Immune cells in the retina demonstrate significant dynamic motility that report on their physiology and function. We have examined the anatomy and behavior of ocular immune cells including 1. microglia in the retina, and 2. macrophages in the choroid. Our work employed ex vivo time-lapse confocal imaging techniques to visualize fluorescence-labeled microglia from transgenic CX3CR1+/GFP mice and follow dynamic microglia behavior in intact retinal and choroidal explants in real time. Immune and vascular alterations in the choroid are implicated in age-related macular degeneration (AMD). As choroidal immune cells are incompletely understood with regard to their physiology and interactions with choroidal vessels, we examined the associations between myeloid and vascular components of the choroid in young and aged mice. Albino CX3CR1(GFP/+) transgenic mice, whose choroidal myeloid cells possess green fluorescence, were perfused intraluminally with the vital dye DiI to label choroidal vessels. The distribution, morphology, behavior, and vascular associations of resident myeloid cells were examined using time-lapse live confocal imaging and immunohistochemical analysis. Dendritiform myeloid cells, comprising most of the resident immune cell population in the choroid, were widely distributed across the choroid and demonstrated close associations with choroidal vessels that varied with their position in the vascular tree. Notably, myeloid cells associated with choroidal arteries and arterioles appeared as elongated cells flanking the long axes of vessels, whereas those associated with the choriocapillaris were distributed as a layer of stellate cells on the scleral but not vitreal choriocapillaris surface. While stationary in position, dendritiform myeloid cells demonstrated the rapid process dynamism well suited to comprehensive immunosurveillance of the perivascular space. Myeloid cells also increased in density as a function of aging, correlating locally with greater choroidal vascular attenuation. Resident myeloid cells demonstrated close but dynamic physical interactions with choroidal vessels, indicative of constitutive immune-vascular interactions in the normal choroid. These interactions may alter progressively with aging, providing a basis for understanding age-related choroidal dysfunction underlying AMD.

视网膜中的免疫细胞表现出显著的动态运动性，这反映了它们的生理和功能。我们已经检查了眼免疫细胞的解剖和行为，包括1。视网膜上的小胶质细胞，2。脉络膜中的巨噬细胞。我们的工作采用离体延时共聚焦成像技术来观察转基因CX3CR1+/GFP小鼠荧光标记的小胶质细胞，并实时跟踪完整视网膜和脉络膜外植体中小胶质细胞的动态行为。