ShEEP Request for Flow Cytometer Special Order Laser Upgrade

摘要 在此应用中，我们要求对现有的Becton Dickinson LSRII流式细胞仪进行以下升级： 1)相干G4 355 nm 60 mw紫外激光器的安装 2)安装在八角形光学阵列上的三个光电倍增管和配置的光学器件： a. BUV396（379/28） B. BUV496（450LP;515/30） C. BUV737（690LP; 740/35） 3)为现有的紫色Octagon添加额外的光电倍增管，用于BV 785（750 LP; 780/60） 4)支持新配置的附加电子设备 5)流式细胞仪流量供应系统 这些升级将使我们可以分析的颜色总数增加到18种，并增强LSRII的 性能能力。升级系统将支持以下研究项目： 1)Marc Goalstone博士，主要研究者：BLR&D Merit Review Award“糖尿病：ERK 5和 血管炎症” 2)Robert基思，医学博士，主要研究者：BLR&D优异评审奖“肺用PPAR γ激动剂 癌症化学预防” 3)Elizabeth Redente，博士，首席研究员：BLR&D职业发展奖“ TNF-α介导的肺纤维化消退” 4)Subbiah Pugazhenthi，博士，主要研究者：BLR&D Merit Review Award“CREB的机制 阿尔茨海默病大脑的失调” 5)Jane Reusch，医学博士，主要研究者：BLR&D Merit Review Award“药理学恢复 糖尿病血管功能障碍 6)丽莎布伦纳，博士，共同首席研究员：CSR&D优秀评论奖“弓形虫， 犬尿氨酸途径和退伍军人的自杀行为” 7)Ehrin Armstrong，医学博士，当地研究中心研究员：CSR&D优异评审奖“斑块消退和 祖细胞动员与强化脂质消除”

Abstract In this application we request the following upgrades to our existing Becton Dickinson LSRII Flow Cytometer: 1) Installation of a Coherent G4 355nm 60mw UV laser 2) Three photomultiplier tubes installed on an Octagon optical array and optics configured: a. BUV396 (379/28) b. BUV496 (450LP;515/30) c. BUV737 (690LP; 740/35) 3) Additional photomultiplier tube added to existing violet Octagon with optics for BV785 (750LP; 780/60) 4) Additional electronics to support the new configuration 5) FACS Flow Supply System These upgrades will increase the number of total colors we can analyze to 18 and enhance the LSRII’s performance capacity. The following research projects will be supported by the upgrades system: 1) Marc Goalstone, PhD, Principal Investigator: BLR&D Merit Review Award “Diabetes: ERK5 and Vascular Inflammation” 2) Robert Keith, MD, Principal Investigator: BLR&D Merit Review Award “PPAR gamma Agonists for Lung Cancer Chemoprevention” 3) Elizabeth Redente, PhD, Principal Investigator: BLR&D Career Development Award “Mechanisms of TNF-alpha Mediated Resolution of Pulmonary Fibrosis” 4) Subbiah Pugazhenthi, PhD, Principal Investigator: BLR&D Merit Review Award “Mechanism of CREB dysregulation in Alzheimer brain” 5) Jane Reusch, MD, Principal Investigator: BLR&D Merit Review Award “Pharmacological Restoration of Diabetic Vascular Dysfunction” 6) Lisa Brenner, PhD, Co-Principal Investigator: CSR&D Merit Review Award “Toxoplasma gondi, the kynurenine pathway, and suicidal behavior in veterans” 7) Ehrin Armstrong, MD, Local Site Investigator: CSR&D Merit Review Award “Plaque Regression and Progenitor Cell Mobilization with Intensive Lipid Elimination”